|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online July 13, 2004
doi: 10.1242/10.1242/jcs.01211
Research Article |
1 Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
2 Analytical Imaging Facility, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
3 Division of Cell Biology, Institute of Ophthalmology, University College London, 11-43 Bath Street, London EC1V 9EL, UK
* Author for correspondence (e-mail: vogniew{at}aecom.yu.edu)
Accepted 10 March 2004
Both the Arp2/3 complex and cofilin are believed to be important for the generation of protrusive force at the leading edge; however, their relative contributions have not been explored in vivo. Our results with living cells show that cofilin enters the leading edge immediately before the start of lamellipod extension, slightly earlier than Arp2/3, which begins to be recruited slightly later as the lamellipod is extended. Blocking either the Arp2/3 complex or cofilin function in cells results in failure to extend broad lamellipods and inhibits free barbed ends, suggesting that neither factor on its own can support actin polymerization-mediated protrusion in response to growth factor stimulation. High-resolution analysis of the actin network at the leading edge supports the idea that both the severing activity of cofilin and the specific branching activity of the Arp2/3 complex are essential for lamellipod protrusion. These results are the first to document the relative contributions of cofilin and Arp2/3 complex in vivo and indicate that cofilin begins to initiate the generation of free barbed ends that act in synergy with the Arp2/3 complex to create a large burst in nucleation activity.
Key words: Cofilin, Arp2/3, Cytoskeleton, Actin
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  J. van Rheenen, J. Condeelis, and M. Glogauer A common cofilin activity cycle in invasive tumor cells and inflammatory cells J. Cell Sci., February 1, 2009; 122(3): 305 - 311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Sarmiento, W. Wang, A. Dovas, H. Yamaguchi, M. Sidani, M. El-Sibai, V. DesMarais, H. A. Holman, S. Kitchen, J. M. Backer, et al. WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells J. Cell Biol., March 24, 2008; 180(6): 1245 - 1260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Kolsch, P. G. Charest, and R. A. Firtel The regulation of cell motility and chemotaxis by phospholipid signaling J. Cell Sci., March 1, 2008; 121(5): 551 - 559. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. van Rheenen, X. Song, W. van Roosmalen, M. Cammer, X. Chen, V. DesMarais, S.-C. Yip, J. M. Backer, R. J. Eddy, and J. S. Condeelis EGF-induced PIP2 hydrolysis releases and activates cofilin locally in carcinoma cells J. Cell Biol., December 17, 2007; 179(6): 1247 - 1259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sidani, D. Wessels, G. Mouneimne, M. Ghosh, S. Goswami, C. Sarmiento, W. Wang, S. Kuhl, M. El-Sibai, J. M. Backer, et al. Cofilin determines the migration behavior and turning frequency of metastatic cancer cells J. Cell Biol., November 19, 2007; 179(4): 777 - 791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. X. Sun, M. A.O. Magalhaes, and M. Glogauer Rac1 and Rac2 differentially regulate actin free barbed end formation downstream of the fMLP receptor J. Cell Biol., October 22, 2007; 179(2): 239 - 245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. El-Sibai, P. Nalbant, H. Pang, R. J. Flinn, C. Sarmiento, F. Macaluso, M. Cammer, J. S. Condeelis, K. M. Hahn, and J. M. Backer Cdc42 is required for EGF-stimulated protrusion and motility in MTLn3 carcinoma cells J. Cell Sci., October 1, 2007; 120(19): 3465 - 3474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-C. Yip, M. El-Sibai, S. J. Coniglio, G. Mouneimne, R. J. Eddy, B. E. Drees, P. O. Neilsen, S. Goswami, M. Symons, J. S. Condeelis, et al. The distinct roles of Ras and Rac in PI 3-kinase-dependent protrusion during EGF-stimulated cell migration J. Cell Sci., September 1, 2007; 120(17): 3138 - 3146. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. de Graauw, S. Le Devedec, I. Tijdens, M. B. Smeets, A. M. Deelder, and B. van de Water Proteomic Analysis of Alternative Protein Tyrosine Phosphorylation in 1,2-Dichlorovinyl-Cysteine-Induced Cytotoxicity in Primary Cultured Rat Renal Proximal Tubular Cells J. Pharmacol. Exp. Ther., July 1, 2007; 322(1): 89 - 100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Wang, J. B. Wyckoff, S. Goswami, Y. Wang, M. Sidani, J. E. Segall, and J. S. Condeelis Coordinated Regulation of Pathways for Enhanced Cell Motility and Chemotaxis Is Conserved in Rat and Mouse Mammary Tumors Cancer Res., April 15, 2007; 67(8): 3505 - 3511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Berkova, S. E. Crawford, S. E. Blutt, A. P. Morris, and M. K. Estes Expression of Rotavirus NSP4 Alters the Actin Network Organization through the Actin Remodeling Protein Cofilin J. Virol., April 1, 2007; 81(7): 3545 - 3553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Dixon and P. J. Roy Muscle arm development in Caenorhabditis elegans Development, July 1, 2005; 132(13): 3079 - 3092. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P. LaLonde, M. C. Brown, B. P. Bouverat, and C. E. Turner Actopaxin Interacts with TESK1 to Regulate Cell Spreading on Fibronectin J. Biol. Chem., June 3, 2005; 280(22): 21680 - 21688. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. H.J. Danen, J. van Rheenen, W. Franken, S. Huveneers, P. Sonneveld, K. Jalink, and A. Sonnenberg Integrins control motile strategy through a Rho-cofilin pathway J. Cell Biol., May 9, 2005; 169(3): 515 - 526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yamaguchi, M. Lorenz, S. Kempiak, C. Sarmiento, S. Coniglio, M. Symons, J. Segall, R. Eddy, H. Miki, T. Takenawa, et al. Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin J. Cell Biol., January 31, 2005; 168(3): 441 - 452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. DesMarais, M. Ghosh, R. Eddy, and J. Condeelis Cofilin takes the lead J. Cell Sci., January 1, 2005; 118(1): 19 - 26. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Mouneimne, L. Soon, V. DesMarais, M. Sidani, X. Song, S.-C. Yip, M. Ghosh, R. Eddy, J. M. Backer, and J. Condeelis Phospholipase C and cofilin are required for carcinoma cell directionality in response to EGF stimulation J. Cell Biol., August 30, 2004; 166(5): 697 - 708. [Abstract] [Full Text] [PDF]
|
|
