spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 29 June 2004
doi: 10.1242/jcs.01231

Journal of Cell Science 117, 3547-3559 (2004)
Published by The Company of Biologists 2004
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Tables
Right arrow All Versions of this Article:
jcs.01231v1
117/16/3547    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JCS
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Petronczki, M.
Right arrow Articles by Nasmyth, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Petronczki, M.
Right arrow Articles by Nasmyth, K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Sister-chromatid cohesion mediated by the alternative RF-CCtf18/Dcc1/Ctf8, the helicase Chl1 and the polymerase-{alpha}-associated protein Ctf4 is essential for chromatid disjunction during meiosis II

Mark Petronczki1,*, Barbara Chwalla1,*, Maria F. Siomos1,*, Shihori Yokobayashi2, Wolfgang Helmhart1, Adam M. Deutschbauer3, Ronald W. Davis3, Yoshinori Watanabe2 and Kim Nasmyth1,{ddagger}

1 Research Institute of Molecular Pathology, Dr. Bohrgasse 7, A-1030 Vienna, Austria
2 Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan
3 Department of Genetics and Department of Biochemistry, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA

{ddagger} Author for correspondence (e-mail: knasmyth{at}imp.univie.ac.at)

Accepted 19 March 2004

Cohesion between sister chromatids mediated by a multisubunit complex called cohesin is established during DNA replication and is essential for the orderly segregation of chromatids during anaphase. In budding yeast, a specialized replication factor C called RF-CCtf18/Dcc1/Ctf8 and the DNA-polymerase-{alpha}-associated protein Ctf4 are required to maintain sister-chromatid cohesion in cells arrested for long periods in mitosis. We show here that CTF8, CTF4 and a helicase encoded by CHL1 are required for efficient sister chromatid cohesion in unperturbed mitotic cells, and provide evidence that Chl1 functions during S-phase. We also show that, in contrast to mitosis, RF-CCtf18/Dcc1/Cft8, Ctf4 and Chl1 are essential for chromosome segregation during meiosis and for the viability of meiotic products. Our finding that cells deleted for CTF8, CTF4 or CHL1 undergo massive meiosis II non-disjunction suggests that the second meiotic division is particularly sensitive to cohesion defects. Using a functional as well as a cytological assay, we demonstrate that CTF8, CHL1 and CTF4 are essential for cohesion between sister centromeres during meiosis but dispensable for cohesin's association with centromeric DNA. Our finding that mutants in fission yeast ctf18 and dcc1 have similar defects suggests that the involvement of the alternative RF-CCtf18/Dcc1/Ctf8 complex in sister chromatid cohesion might be highly conserved.

Key words: Sister-chromatid cohesion, Mitosis, Meiosis


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related articles in JCS:

Cohesion's complexity

JCS 2004 117: 1604. [Full Text]  



This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
A. R. Leman, C. Noguchi, C. Y. Lee, and E. Noguchi
Human Timeless and Tipin stabilize replication forks and facilitate sister-chromatid cohesion
J. Cell Sci., March 1, 2010; 123(5): 660 - 670.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
K. H. Schmidt, E. B. Viebranz, L. B. Harris, H. Mirzaei-Souderjani, S. Syed, and R. Medicus
Defects in DNA Lesion Bypass Lead to Spontaneous Chromosomal Rearrangements and Increased Cell Death
Eukaryot. Cell, February 1, 2010; 9(2): 315 - 324.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Yoshizawa-Sugata and H. Masai
Roles of Human AND-1 in Chromosome Transactions in S Phase
J. Biol. Chem., July 31, 2009; 284(31): 20718 - 20728.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
H. Tanaka, Y. Katou, M. Yagura, K. Saitoh, T. Itoh, H. Araki, M. Bando, and K. Shirahige
Ctf4 coordinates the progression of helicase and DNA polymerase {alpha}
Genes Cells, July 1, 2009; 14(7): 807 - 820.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
J.-M. Peters, A. Tedeschi, and J. Schmitz
The cohesin complex and its roles in chromosome biology
Genes & Dev., November 15, 2008; 22(22): 3089 - 3114.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
T. R. Ben-Shahar, S. Heeger, C. Lehane, P. East, H. Flynn, M. Skehel, and F. Uhlmann
Eco1-Dependent Cohesin Acetylation During Establishment of Sister Chromatid Cohesion
Science, July 25, 2008; 321(5888): 563 - 566.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Farina, J.-H. Shin, D.-H. Kim, V. P. Bermudez, Z. Kelman, Y.-S. Seo, and J. Hurwitz
Studies with the Human Cohesin Establishment Factor, ChlR1: ASSOCIATION OF ChlR1 WITH Ctf18-RFC AND Fen1
J. Biol. Chem., July 25, 2008; 283(30): 20925 - 20936.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
K. M. Berkowitz, K. H. Kaestner, and T. A. Jongens
Germline expression of mammalian CTF18, an evolutionarily conserved protein required for germ cell proliferation in the fly and sister chromatid cohesion in yeast
Mol. Hum. Reprod., March 1, 2008; 14(3): 143 - 150.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. B. Ansbach, C. Noguchi, I. W. Klansek, M. Heidlebaugh, T. M. Nakamura, and E. Noguchi
RFCCtf18 and the Swi1-Swi3 Complex Function in Separate and Redundant Pathways Required for the Stabilization of Replication Forks to Facilitate Sister Chromatid Cohesion in Schizosaccharomyces pombe
Mol. Biol. Cell, February 1, 2008; 19(2): 595 - 607.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
W. Zhu, C. Ukomadu, S. Jha, T. Senga, S. K. Dhar, J. A. Wohlschlegel, L. K. Nutt, S. Kornbluth, and A. Dutta
Mcm10 and And-1/CTF4 recruit DNA polymerase {alpha} to chromatin for initiation of DNA replication
Genes & Dev., September 15, 2007; 21(18): 2288 - 2299.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. V. Skibbens, M. Maradeo, and L. Eastman
Fork it over: the cohesion establishment factor Ctf7p and DNA replication
J. Cell Sci., August 1, 2007; 120(15): 2471 - 2477.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
H. Xu, C. Boone, and G. W. Brown
Genetic Dissection of Parallel Sister-Chromatid Cohesion Pathways
Genetics, July 1, 2007; 176(3): 1417 - 1429.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
C. C. Reis and J. L. Campbell
Contribution of Trf4/5 and the Nuclear Exosome to Genome Stability Through Regulation of Histone mRNA Levels in Saccharomyces cerevisiae
Genetics, March 1, 2007; 175(3): 993 - 1010.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. L. Parish, J. Rosa, X. Wang, J. M. Lahti, S. J. Doxsey, and E. J. Androphy
The DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells
J. Cell Sci., December 1, 2006; 119(23): 4857 - 4865.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Laha, S. P. Das, S. Hajra, S. Sau, and P. Sinha
The budding yeast protein Chl1p is required to preserve genome integrity upon DNA damage in S-phase
Nucleic Acids Res., November 6, 2006; 34(20): 5880 - 5891.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
E. Coic, K. Sun, C. Wu, and J. E. Haber
Cell Cycle-Dependent Regulation of Saccharomyces cerevisiae Donor Preference during Mating-Type Switching by SBF (Swi4/Swi6) and Fkh1
Mol. Cell. Biol., July 15, 2006; 26(14): 5470 - 5480.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L. Haracska, R. E. Johnson, L. Prakash, and S. Prakash
Trf4 and Trf5 Proteins of Saccharomyces cerevisiae Exhibit Poly(A) RNA Polymerase Activity but No DNA Polymerase Activity
Mol. Cell. Biol., November 15, 2005; 25(22): 10183 - 10189.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Kim, K. Robertson, K. J. L. Mylonas, F. C. Gray, I. Charapitsa, and S. A. MacNeill
Contrasting effects of Elg1-RFC and Ctf18-RFC inactivation in the absence of fully functional RFC in fission yeast
Nucleic Acids Res., July 21, 2005; 33(13): 4078 - 4089.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
G. O. Bylund and P. M. J. Burgers
Replication Protein A-Directed Unloading of PCNA by the Ctf18 Cohesion Establishment Complex
Mol. Cell. Biol., July 1, 2005; 25(13): 5445 - 5455.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
R. V. Skibbens
Unzipped and loaded: the role of DNA helicases and RFC clamp-loading complexes in sister chromatid cohesion
J. Cell Biol., June 20, 2005; 169(6): 841 - 846.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Zhou and T. S.-F. Wang
A Coordinated Temporal Interplay of Nucleosome Reorganization Factor, Sister Chromatin Cohesion Factor, and DNA Polymerase {alpha} Facilitates DNA Replication
Mol. Cell. Biol., November 1, 2004; 24(21): 9568 - 9579.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004