|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online July 13, 2004
doi: 10.1242/10.1242/jcs.01214
Research Article |
1 Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
2 ARC Centre of Excellence in Biotechnology and Development, School of Environmental and Life Sciences, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
* Author for correspondence (e-mail: jaitken{at}mail.newcastle.edu.au)
Accepted 10 March 2004
Mammalian spermatozoa undergo a series of molecular and biochemical changes collectively termed capacitation prior to acquiring the ability to fertilise the oocyte. Although phosphorylation of sperm proteins on tyrosine residues has been recognised as an important component of this process, the precise relationship between the phosphorylation status of mammalian spermatozoa and their capacity for fertilisation has remained unclear. In this study we demonstrate a causal relationship between tyrosine phosphorylation in spermatozoa and sperm-zona interaction. The phosphotyrosine expression associated with sperm capacitation localised to internal flagellar structures in permeabilised cells but could also be detected on the exterior surface of the sperm head in live cells. Importantly, almost all spermatozoa bound to the zona pellucida demonstrated this pattern of phosphoprotein localisation, compared to fewer than 15% of the free-swimming population. These data suggest that tyrosine phosphorylation plays a significant role in remodelling the sperm surface, so that these cells are able to recognise the zona pellucida. Phosphoproteome analysis yielded the first evidence of molecular chaperones, endoplasmin (erp99) and heat shock protein 60 (hsp60), as targets for phosphorylation on the surface of mouse spermatozoa, whereas immunofluorescence localised these proteins to the precise region of the sperm head that participates in zona recognition. Based on these results, we propose a novel mechanism for mammalian gamete interaction whereby the activation of sperm-surface chaperones by tyrosine phosphorylation during capacitation may trigger conformational changes facilitating the formation of a functional zona pellucida receptor complex on the surface of mammalian spermatozoa.
Key words: Fertilisation, Signal Transduction, Sperm, Capacitation, Phosphorylation, Chaperone
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
Related articles in JCS:
This article has been cited by other articles:
|
|
 |
|
  M.-K. Chung, P. C.N. Chiu, C.-L. Lee, R. T.K. Pang, E.H.Y. Ng, K.-F. Lee, R. Koistinen, H. Koistinen, M. Seppala, and W. S.B. Yeung Cumulus-associated {alpha}2-macroglobulin derivative retains proconceptive glycodelin-C in the human cumulus matrix Hum. Reprod., November 1, 2009; 24(11): 2856 - 2867. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Borg, K. M. Wolski, G. M. Gibbs, and M. K. O'Bryan Phenotyping male infertility in the mouse: how to get the most out of a 'non-performer' Hum. Reprod. Update, September 15, 2009; (2009) dmp032v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Grasa, C. Colas, M. Gallego, L. Monteagudo, T. Muino-Blanco, and J. A. Cebrian-Perez Changes in content and localization of proteins phosphorylated at tyrosine, serine and threonine residues during ram sperm capacitation and acrosome reaction Reproduction, April 1, 2009; 137(4): 655 - 667. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Maravilla-Galvan, R. Fierro, H. Gonzalez-Marquez, S. Gomez-Arroyo, I. Jimenez, and M. Betancourt Effects of Atrazine and Fenoxaprop-Ethyl on Capacitation and the Acrosomal Reaction in Boar Sperm International Journal of Toxicology, January 1, 2009; 28(1): 24 - 32. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.Y. Liu, M.L. Liu, and H.W.G. Baker Enhancement of sperm-zona pellucida (ZP) binding capacity by activation of protein kinase A and C pathways in certain infertile men with defective sperm-ZP binding Hum. Reprod., January 1, 2009; 24(1): 20 - 27. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Gibbs, K. Roelants, and M. K. O'Bryan The CAP Superfamily: Cysteine-Rich Secretory Proteins, Antigen 5, and Pathogenesis-Related 1 Proteins--Roles in Reproduction, Cancer, and Immune Defense Endocr. Rev., December 1, 2008; 29(7): 865 - 897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Varano, A. Lombardi, G. Cantini, G. Forti, E. Baldi, and M. Luconi Src activation triggers capacitation and acrosome reaction but not motility in human spermatozoa Hum. Reprod., December 1, 2008; 23(12): 2652 - 2662. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Lawson, S. Goupil, and P. Leclerc Increased Activity of the Human Sperm Tyrosine Kinase SRC by the cAMP-Dependent Pathway in the Presence of Calcium Biol Reprod, October 1, 2008; 79(4): 657 - 666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Walsh, D. Whelan, A. Bielanowicz, B. Skinner, R. J. Aitken, M. K. O'Bryan, and B. Nixon Identification of the Molecular Chaperone, Heat Shock Protein 1 (Chaperonin 10), in the Reproductive Tract and in Capacitating Spermatozoa in the Male Mouse Biol Reprod, June 1, 2008; 78(6): 983 - 993. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Mitchell, B. Nixon, M. A. Baker, and R. J. Aitken Investigation of the role of SRC in capacitation-associated tyrosine phosphorylation of human spermatozoa Mol. Hum. Reprod., April 1, 2008; 14(4): 235 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.A. Mitchell, B. Nixon, and R.J. Aitken Analysis of chaperone proteins associated with human spermatozoa during capacitation Mol. Hum. Reprod., September 1, 2007; 13(9): 605 - 613. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. C. N. Chiu, M.-K. Chung, R. Koistinen, H. Koistinen, M. Seppala, P.-C. Ho, E. H. Y. Ng, K.-F. Lee, and W. S. B. Yeung Cumulus Oophorus-associated Glycodelin-C Displaces Sperm-bound Glycodelin-A and -F and Stimulates Spermatozoa-Zona Pellucida Binding J. Biol. Chem., February 23, 2007; 282(8): 5378 - 5388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lin, Y. H. Lee, W. Xu, M. A. Baker, and R. J. Aitken Ontogeny of Tyrosine Phosphorylation-Signaling Pathways During Spermatogenesis and Epididymal Maturation in the Mouse Biol Reprod, October 1, 2006; 75(4): 588 - 597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Baker, L. Hetherington, and R. J. Aitken Identification of SRC as a key PKA-stimulated tyrosine kinase involved in the capacitation-associated hyperactivation of murine spermatozoa J. Cell Sci., August 1, 2006; 119(15): 3182 - 3192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.Y. Liu, G.N. Clarke, and H.W.G. Baker Tyrosine phosphorylation on capacitated human sperm tail detected by immunofluorescence correlates strongly with sperm-zona pellucida (ZP) binding but not with the ZP-induced acrosome reaction Hum. Reprod., April 1, 2006; 21(4): 1002 - 1008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Nixon, D. A. MacIntyre, L. A. Mitchell, G. M. Gibbs, M. O'Bryan, and R. J. Aitken The Identification of Mouse Sperm-Surface-Associated Proteins and Characterization of Their Ability to Act as Decapacitation Factors Biol Reprod, February 1, 2006; 74(2): 275 - 287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cotton, G. M. Gibbs, L. G. Sanchez-Partida, J. R. Morrison, D. M. de Kretser, and M. K. O'Bryan FGFR-1 signaling is involved in spermiogenesis and sperm capacitation J. Cell Sci., January 1, 2006; 119(1): 75 - 84. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Nixon, J. W. Paul, C. M. Spiller, A. G. Attwell-Heap, L. K. Ashman, and R. J. Aitken Evidence for the involvement of PECAM-1 in a receptor mediated signal-transduction pathway regulating capacitation-associated tyrosine phosphorylation in human spermatozoa J. Cell Sci., October 15, 2005; 118(20): 4865 - 4877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Bedu-Addo, L. Lefievre, F.L.C. Moseley, C.L.R. Barratt, and S.J. Publicover Bicarbonate and bovine serum albumin reversibly 'switch' capacitation-induced events in human spermatozoa Mol. Hum. Reprod., September 1, 2005; 11(9): 683 - 691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Furimsky, N. Vuong, H. Xu, P. Kumarathasan, M. Xu, W. Weerachatyanukul, M. Bou Khalil, M. Kates, and N. Tanphaichitr Percoll Gradient-Centrifuged Capacitated Mouse Sperm Have Increased Fertilizing Ability and Higher Contents of Sulfogalactosylglycerolipid and Docosahexaenoic Acid-Containing Phosphatidylcholine Compared to Washed Capacitated Mouse Sperm Biol Reprod, March 1, 2005; 72(3): 574 - 583. [Abstract] [Full Text] [PDF]
|
|
