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First published online 3 August 2004
doi: 10.1242/jcs.01200

Journal of Cell Science 117, 4265-4275 (2004)
Published by The Company of Biologists 2004
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Research Article

AtSGP1, AtSGP2 and MAP4K{alpha} are nucleolar plant proteins that can complement fission yeast mutants lacking a functional SIN pathway

Antony Champion1,*,¶, Stefan Jouannic1,{ddagger}, Stéfanie Guillon1, Keithanne Mockaitis1,§, Andrea Krapp2, Alain Picaud1, Viesturs Simanis2, Martin Kreis1 and Yves Henry1

1 Institut de Biotechnologie des Plantes (IBP), UMR 8618, Bâtiment 630, Université de Paris-Sud, 91405 Orsay Cedex, France
2 Cell Cycle Control Laboratory, ISREC, Chemin des Boveresses 155, Epalinges, 1066, Switzerland

Author for correspondence (e-mail: champion{at}rulbim.leidenuniv.nl)

Accepted 8 March 2004

In the fission yeast Schizosaccharomyces pombe, the onset of septum formation is signalled via the septation initiation network (SIN) involving several protein kinases and a GTPase. Arabidopsis thaliana and Brassica napus proteins homologous to fission yeast spg1p (AtSGP1, AtSGP2), cdc7p (AtMAP3K{epsilon}1, AtMAP3K{epsilon}2, BnMAP3K{epsilon}1) and sid1p (AtMAP4K{alpha}1, AtMAP4K{alpha}2, BnMAP4K{alpha}2) exhibit a significant similarity. The plant proteins AtSGP1/2 and BnMAP4K{alpha}2 are able to complement the S. pombe mutant proteins spg1-B8 and sid1-239, respectively and to induce mutisepta when overexpressed in wild-type yeast. Yeast two-hybrid assays demonstrated interactions both between plant proteins and between plant and yeast proteins of the SIN pathway. However, the primary structure of the proteins as well as the partial complementation of yeast mutants indicates that plant homologous proteins and their yeast counterparts have diverged during evolution. Real-time RT-PCR studies demonstrated plant SIN-related gene expression in all organs tested and a co-expression pattern during the cell cycle, with a higher accumulation at G2-M. During interphase, the plant SIN-related proteins were found to co-localise predominantly in the nucleolus of the plant cells, as shown by fusions to green fluorescent protein. These data suggest the existence of a plant SIN-related pathway.

Key words: Cell cycle, Cytokinesis, Kinase, Plant, Signalling


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© The Company of Biologists Ltd 2004