QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 10 August 2004
doi: 10.1242/jcs.01300

This Article Figures Only Full Text Full Text (PDF) All Versions of this Article: jcs.01300v1 117/19/4401    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Excoffon, K. J. D. A. Articles by Zabner, J. Search for Related Content PubMed PubMed Citation Articles by Excoffon, K. J. D. A. Articles by Zabner, J. Social Bookmarking                         What's this?

A role for the PDZ-binding domain of the coxsackie B virus and adenovirus receptor (CAR) in cell adhesion and growth

¹ Department of Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, 200 Hawkins Drive, Iowa City, IA 52242, USA
² Howard Hughes Medical Institute, University of Iowa, 200 Hawkins Drive, Iowa City, IA 52242, USA

^* Author for correspondence (e-mail: joseph-zabner{at}uiowa.edu)

Accepted 4 May 2004

The coxsackie and adenovirus receptor (CAR) plays a role in viral infection, maintenance of the junction adhesion complex in polarized epithelia, and modulation of cellular growth properties. As a viral receptor, the C-terminus appears to play no role indicating that the major function of CAR is to tether the virus to the cell. By contrast, the C-terminus is known to play a role in cellular localization and probably has a significant function in CAR-mediated adhesion and cell growth properties. We hypothesized that the CAR PDZ (PSD-95/Disc-large/ZO-1) binding motif interacts with PDZ-domain-containing proteins to modulate the cellular phenotype. CAR was modified by deleting the last four amino acids (CAR^GSIV) and evaluated for cell-cell adhesion in polarized primary human airway epithelia and growth characteristics in stably transfected L-cells. Although ablation of the CAR PDZ-binding motif did not affect adenoviral infection, it did have a significant effect both on cell-cell adhesion and on cell growth. Expression of CAR^GSIV failed to increase the transepithelial resistance in polarized epithelia to the same degree as wild-type CAR and failed to act as a growth modulator in L-cells. Furthermore, we provide evidence for three new CAR interacting partners, including MAGI-1b, PICK1 and PSD-95. CAR appears to interact with several distinct PDZ-domain-containing proteins and may exert its biological function through these interactions.

Key words: PDZ-binding motif, Adenovirus receptor, Cell adhesion

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				P. F. Bradfield, S. Nourshargh, M. Aurrand-Lions, and B. A. Imhof JAM Family and Related Proteins in Leukocyte Migration (Vestweber Series) Arterioscler. Thromb. Vasc. Biol., October 1, 2007; 27(10): 2104 - 2112. [Abstract] [Full Text] [PDF]

				H. Cui, A. Hayashi, H.-S. Sun, M. P. Belmares, C. Cobey, T. Phan, J. Schweizer, M. W. Salter, Y. T. Wang, R. A. Tasker, et al. PDZ Protein Interactions Underlying NMDA Receptor-Mediated Excitotoxicity and Neuroprotection by PSD-95 Inhibitors J. Neurosci., September 12, 2007; 27(37): 9901 - 9915. [Abstract] [Full Text] [PDF]

				K. J. D. A. Excoffon, N. Gansemer, G. Traver, and J. Zabner Functional Effects of Coxsackievirus and Adenovirus Receptor Glycosylation on Homophilic Adhesion and Adenoviral Infection J. Virol., June 1, 2007; 81(11): 5573 - 5578. [Abstract] [Full Text] [PDF]

				P. T. Fok, K.-C. Huang, P. C. Holland, and J. Nalbantoglu The Coxsackie and Adenovirus Receptor Binds Microtubules and Plays a Role in Cell Migration J. Biol. Chem., March 9, 2007; 282(10): 7512 - 7521. [Abstract] [Full Text] [PDF]

				C. A. Shaw, N. Larochelle, R. W.R. Dudley, H. Lochmuller, G. Danialou, B. J. Petrof, G. Karpati, P. C. Holland, and J. Nalbantoglu Simultaneous Dystrophin and Dysferlin Deficiencies Associated with High-Level Expression of the Coxsackie and Adenovirus Receptor in Transgenic Mice Am. J. Pathol., December 1, 2006; 169(6): 2148 - 2160. [Abstract] [Full Text] [PDF]

				J.-W. Chen, B. Zhou, Q.-C. Yu, S. J. Shin, K. Jiao, M. D. Schneider, H. S. Baldwin, and J. M. Bergelson Cardiomyocyte-Specific Deletion of the Coxsackievirus and Adenovirus Receptor Results in Hyperplasia of the Embryonic Left Ventricle and Abnormalities of Sinuatrial Valves Circ. Res., April 14, 2006; 98(7): 923 - 930. [Abstract] [Full Text] [PDF]

				A. A. Dorner, F. Wegmann, S. Butz, K. Wolburg-Buchholz, H. Wolburg, A. Mack, I. Nasdala, B. August, J. Westermann, F. G. Rathjen, et al. Coxsackievirus-adenovirus receptor (CAR) is essential for early embryonic cardiac development J. Cell Sci., August 1, 2005; 118(15): 3509 - 3521. [Abstract] [Full Text] [PDF]

				K. J. D. A. Excoffon, G. L. Traver, and J. Zabner The Role of the Extracellular Domain in the Biology of the Coxsackievirus and Adenovirus Receptor Am. J. Respir. Cell Mol. Biol., June 1, 2005; 32(6): 498 - 503. [Abstract] [Full Text] [PDF]

				K. Zen, Y. Liu, I. C. McCall, T. Wu, W. Lee, B. A. Babbin, A. Nusrat, and C. A. Parkos Neutrophil Migration across Tight Junctions Is Mediated by Adhesive Interactions between Epithelial Coxsackie and Adenovirus Receptor and a Junctional Adhesion Molecule-like Protein on Neutrophils Mol. Biol. Cell, June 1, 2005; 16(6): 2694 - 2703. [Abstract] [Full Text] [PDF]

				K. L. Madsen, T. Beuming, M. Y. Niv, C.-w. Chang, K. K. Dev, H. Weinstein, and U. Gether Molecular Determinants for the Complex Binding Specificity of the PDZ Domain in PICK1 J. Biol. Chem., May 27, 2005; 280(21): 20539 - 20548. [Abstract] [Full Text] [PDF]