spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 17 August 2004
doi: 10.1242/jcs.01331

Journal of Cell Science 117, 4559-4569 (2004)
Published by The Company of Biologists 2004
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
jcs.01331v1
117/19/4559    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vouret-Craviari, V.
Right arrow Articles by Van Obberghen-Schilling, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vouret-Craviari, V.
Right arrow Articles by Van Obberghen-Schilling, E.

Research Article

ILK is required for the assembly of matrix-forming adhesions and capillary morphogenesis in endothelial cells

Valérie Vouret-Craviari*, Etienne Boulter*, Dominique Grall, Cédric Matthews and Ellen Van Obberghen-Schilling{ddagger}

Institute of Signaling, Developmental Biology and Cancer Research CNRS-UMR6543, Centre Antoine Lacassagne, 33 Avenue de Valombrose, 06189 Nice, France

{ddagger} Author for correspondence (e-mail: vanobber{at}unice.fr)

Accepted 26 May 2004

Integrins play a key role in regulating endothelial cell survival, migration and differentiated function during angiogenic blood-vessel remodeling. Integrin-linked kinase (ILK) is a multidomain protein that interacts with the cytoplasmic tail of integrin ß subunits and is thought to participate in integrin-mediated signal transduction. We report here that attenuation of ILK expression in cultured bovine aortic endothelial cells by RNA interference had marked effects on surface distribution of {alpha}5ß1 integrin and the organization of cell-matrix adhesions characterized by the disappearance of fibrillar (3D-like) adhesions that are rich in {alpha}5ß1 and paxillin, and associated fibrillar fibronectin matrix. This defect was not caused by a decrease in fibronectin mRNA levels or by intracellular retention of the protein. Adhesion to surface-adsorbed matrix proteins based on ß1 and ß3 integrin was enhanced following ILK depletion, whereas cell spreading, migration and multilayer alignment into capillary-like structures on Matrigel were impaired. We conclude that ILK is an important regulator of the endothelial phenotype and vascular network formation by directing the assembly and/or maturation of {alpha}5ß1-competent matrix-forming adhesions.

Key words: Integrin-linked kinase, Endothelial cells, Fibronectin fibrillogenesis, Adhesion, Migration




This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
B. Ho, G. Hou, J. G. Pickering, G. Hannigan, B. L. Langille, and M. P. Bendeck
Integrin-Linked Kinase in the Vascular Smooth Muscle Cell Response to Injury
Am. J. Pathol., July 1, 2008; 173(1): 278 - 288.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
M. B. Joshi, D. Ivanov, M. Philippova, P. Erne, and T. J. Resink
Integrin-linked kinase is an essential mediator for T-cadherin-dependent signaling via Akt and GSK3{beta} in endothelial cells
FASEB J, October 1, 2007; 21(12): 3083 - 3095.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J.-S. Mo, M.-Y. Kim, S.-O. Han, I.-S. Kim, E.-J. Ann, K. S. Lee, M.-S. Seo, J.-Y. Kim, S.-C. Lee, J.-W. Park, et al.
Integrin-Linked Kinase Controls Notch1 Signaling by Down-Regulation of Protein Stability through Fbw7 Ubiquitin Ligase
Mol. Cell. Biol., August 1, 2007; 27(15): 5565 - 5574.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Yoneda, D. Ushakov, H. A.B. Multhaupt, and J. R. Couchman
Fibronectin Matrix Assembly Requires Distinct Contributions from Rho Kinases I and -II
Mol. Biol. Cell, January 1, 2007; 18(1): 66 - 75.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
E. Boulter, D. Grall, S. Cagnol, and E. Van Obberghen-Schilling
Regulation of cell-matrix adhesion dynamics and Rac-1 by integrin linked kinase
FASEB J, July 1, 2006; 20(9): 1489 - 1491.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. Varon, F. Tatin, V. Moreau, E. Van Obberghen-Schilling, S. Fernandez-Sauze, E. Reuzeau, I. Kramer, and E. Genot
Transforming Growth Factor {beta} Induces Rosettes of Podosomes in Primary Aortic Endothelial Cells.
Mol. Cell. Biol., May 1, 2006; 26(9): 3582 - 3594.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
C. El-Aouni, N. Herbach, S. M. Blattner, A. Henger, M. P. Rastaldi, G. Jarad, J. H. Miner, M. J. Moeller, R. St-Arnaud, S. Dedhar, et al.
Podocyte-Specific Deletion of Integrin-Linked Kinase Results in Severe Glomerular Basement Membrane Alterations and Progressive Glomerulosclerosis
J. Am. Soc. Nephrol., May 1, 2006; 17(5): 1334 - 1344.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. A. K. M. Khyrul, D. P. LaLonde, M. C. Brown, H. Levinson, and C. E. Turner
The Integrin-linked Kinase Regulates Cell Morphology and Motility in a Rho-associated Kinase-dependent Manner
J. Biol. Chem., December 24, 2004; 279(52): 54131 - 54139.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004