|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
doi: 10.1242/jcs.00854
Research Article |
1 Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN, Saitama 351-0198, Japan
2 Division of Molecular Neurobiology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
3 Department of Pediatrics, Tokyo Women's Medical University, Tokyo 162-8666, Japan
4 Calcium Oscillation Project, ICORP, Japan Science and Technology Corporation (JST), Tokyo 108-0071, Japan
* Author for correspondence (e-mail: tinoue{at}ims.u-tokyo.ac.jp)
Accepted 4 September 2003
Although spatially restricted Ca2+ release from the endoplasmic reticulum (ER) through intracellular Ca2+ channels plays important roles in various neuronal activities, the accurate distribution and dynamics of ER in the dendrite of living neurons still remain unknown. To elucidate these, we expressed fluorescent protein-tagged ER proteins in cultured mouse hippocampal neurons, and monitored their movements using time-lapse microscopy. We report here that a sub-compartment of ER forms in relatively large vesicles that are capable, similarly to the reticular ER, of taking up and releasing Ca2+. The vesicular sub-compartment of ER moved rapidly along the dendrites in both anterograde and retrograde directions at a velocity of 0.2-0.3 µm/second. Depletion of microtubules, overexpression of dominant-negative kinesin and kinesin depletion by antisense DNA reduced the number and velocity of the moving vesicles, suggesting that kinesin may drive the transport of the vesicular sub-compartment of ER along microtubules in the dendrite. Rapid transport of the Ca2+-releasable sub-compartment of ER might contribute to rapid supply of fresh ER proteins to the distal part of the dendrite, or to the spatial regulation of intracellular Ca2+ signaling.
Key words: Endoplasmic reticulum, Ins(1,4,5)P3R, Neuron, Dendrite, Kinesin, Microtubule
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  M. J. Wozniak, B. Bola, K. Brownhill, Y.-C. Yang, V. Levakova, and V. J. Allan Role of kinesin-1 and cytoplasmic dynein in endoplasmic reticulum movement in VERO cells J. Cell Sci., June 15, 2009; 122(12): 1979 - 1989. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Fuentes and C. O. Arregui Microtubule and Cell Contact Dependency of ER-bound PTP1B Localization in Growth Cones Mol. Biol. Cell, March 15, 2009; 20(6): 1878 - 1889. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. K. Shrum, D. DeFrancisco, and M. K. Meffert Stimulated nuclear translocation of NF-{kappa}B and shuttling differentially depend on dynein and the dynactin complex PNAS, February 24, 2009; 106(8): 2647 - 2652. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. McCarron and M. L. Olson A Single Luminally Continuous Sarcoplasmic Reticulum with Apparently Separate Ca2+ Stores in Smooth Muscle J. Biol. Chem., March 14, 2008; 283(11): 7206 - 7218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Nakano, T. Kohno, T. Hibi, S. Kohno, A. Baba, K. Mikoshiba, K. Nakajima, and M. Hattori The Extremely Conserved C-terminal Region of Reelin Is Not Necessary for Secretion but Is Required for Efficient Activation of Downstream Signaling J. Biol. Chem., July 13, 2007; 282(28): 20544 - 20552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Mironov and N. Symonchuk ER vesicles and mitochondria move and communicate at synapses J. Cell Sci., December 1, 2006; 119(23): 4926 - 4934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Baba, K. Hayashi, Y. Fujii, A. Mizushima, H. Watarai, M. Wakamori, T. Numaga, Y. Mori, M. Iino, M. Hikida, et al. Coupling of STIM1 to store-operated Ca2+ entry through its constitutive and inducible movement in the endoplasmic reticulum PNAS, November 7, 2006; 103(45): 16704 - 16709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Sannerud, M. Marie, C. Nizak, H. A. Dale, K. Pernet-Gallay, F. Perez, B. Goud, and J. Saraste Rab1 Defines a Novel Pathway Connecting the Pre-Golgi Intermediate Compartment with the Cell Periphery Mol. Biol. Cell, April 1, 2006; 17(4): 1514 - 1526. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Taylor and J. R. Fallon Dendrites Contain a Spacing Pattern J. Neurosci., January 25, 2006; 26(4): 1154 - 1163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Rizzuto and T. Pozzan Microdomains of Intracellular Ca2+: Molecular Determinants and Functional Consequences Physiol Rev, January 1, 2006; 86(1): 369 - 408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Poteryaev, J. M. Squirrell, J. M. Campbell, J. G. White, and A. Spang Involvement of the Actin Cytoskeleton and Homotypic Membrane Fusion in ER Dynamics in Caenorhabditis elegans Mol. Biol. Cell, May 1, 2005; 16(5): 2139 - 2153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Farah, D. Liazoghli, S. Perreault, M. Desjardins, A. Guimont, A. Anton, M. Lauzon, G. Kreibich, J. Paiement, and N. Leclerc Interaction of Microtubule-associated Protein-2 and p63: A NEW LINK BETWEEN MICROTUBULES AND ROUGH ENDOPLASMIC RETICULUM MEMBRANES IN NEURONS J. Biol. Chem., March 11, 2005; 280(10): 9439 - 9449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Bannai, K. Fukatsu, A. Mizutani, T. Natsume, S.-i. Iemura, T. Ikegami, T. Inoue, and K. Mikoshiba An RNA-interacting Protein, SYNCRIP (Heterogeneous Nuclear Ribonuclear Protein Q1/NSAP1) Is a Component of mRNA Granule Transported with Inositol 1,4,5-Trisphosphate Receptor Type 1 mRNA in Neuronal Dendrites J. Biol. Chem., December 17, 2004; 279(51): 53427 - 53434. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Fukatsu, H. Bannai, S. Zhang, H. Nakamura, T. Inoue, and K. Mikoshiba Lateral Diffusion of Inositol 1,4,5-Trisphosphate Receptor Type 1 Is Regulated by Actin Filaments and 4.1N in Neuronal Dendrites J. Biol. Chem., November 19, 2004; 279(47): 48976 - 48982. [Abstract] [Full Text] [PDF]
|
|
