|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online December 15, 2003
doi: 10.1242/10.1242/jcs.00839
Research Article |
Institute of Molecular Biology, Department of Cell Biology, Austrian Academy of Sciences, Billrothstrasse 11, 5020 Salzburg, Austria
* Author for correspondence (e-mail:mgimona{at}imb.oeaw.ac.at)
Accepted 19 August 2003
Activation of conventional protein kinase C by phorbol ester triggers the Src-dependent remodelling of the actin cytoskeleton and the formation of podosomes in vascular smooth muscle cells. Rearrangement of actin cytoskeleton in response to phorbol-12,13-dibutyrate is characterised by the simultaneous disassembly of peripheral actin stress fibres and focal adhesions, focal de novo actin polymerisation and actomyosin contraction in the cell center, indicating a spatially and temporally segregated, differential modulation of actin-cytoskeleton stability and turnover. Taking advantage of the prominent actin cytoskeleton in A7r5 cells we show here, that the molecular basis for the local inhibition of contractility is the specific recruitment of p190RhoGAP to specialised microdomains at the focal adhesion/stress fibre interface, which are constitutively enriched in cortactin. The microdomains contain structurally altered actin filaments inaccessible to phalloidin. However, the filaments remain decorated with high molecular weight tropomyosins. Clustering of cortactin during podosome formation causes the rapid, local dispersion of myosin and tropomyosin, and interferes with the F-actin binding of h1calponin, consistent with a RhoGAP-mediated reduction of contractility. Phorbol ester-induced podosome formation is efficiently blocked by expression of constitutively active Dia1, which leads to the dispersion of cortactin. The results provide direct evidence for the spatially restricted inhibition of contractility via the recruitment and accumulation of cortactin and p190RhoGAP.
Key words: p190RhoGAP, Smooth muscle, Myosin, Cortactin, Podosomes
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
Related articles in JCS:
This article has been cited by other articles:
|
|
 |
|
  C. Albiges-Rizo, O. Destaing, B. Fourcade, E. Planus, and M. R. Block Actin machinery and mechanosensitivity in invadopodia, podosomes and focal adhesions J. Cell Sci., September 1, 2009; 122(17): 3037 - 3049. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-T. Zhu, Y. Hayashida, A. Kheirkhah, H. He, S.-Y. Chen, and S. C. G. Tseng Characterization and Comparison of Intercellular Adherent Junctions Expressed by Human Corneal Endothelial Cells In Vivo and In Vitro Invest. Ophthalmol. Vis. Sci., September 1, 2008; 49(9): 3879 - 3886. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Gunst and W. Zhang Actin cytoskeletal dynamics in smooth muscle: a new paradigm for the regulation of smooth muscle contraction Am J Physiol Cell Physiol, September 1, 2008; 295(3): C576 - C587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Dorfleutner, Y. Cho, D. Vincent, J. Cunnick, H. Lin, S. A. Weed, C. Stehlik, and D. C. Flynn Phosphorylation of AFAP-110 affects podosome lifespan in A7r5 cells J. Cell Sci., July 15, 2008; 121(14): 2394 - 2405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Guegan, F. Tatin, T. Leste-Lasserre, G. Drutel, E. Genot, and V. Moreau p190B RhoGAP regulates endothelial-cell-associated proteolysis through MT1-MMP and MMP2 J. Cell Sci., June 15, 2008; 121(12): 2054 - 2061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. F. G. van Helden, M. M. Oud, B. Joosten, N. Peterse, C. G. Figdor, and F. N. van Leeuwen PGE2-mediated podosome loss in dendritic cells is dependent on actomyosin contraction downstream of the RhoA-Rho-kinase axis J. Cell Sci., April 1, 2008; 121(7): 1096 - 1106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Gunning, G. O'neill, and E. Hardeman Tropomyosin-Based Regulation of the Actin Cytoskeleton in Time and Space Physiol Rev, January 1, 2008; 88(1): 1 - 35. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Boguslavsky, I. Grosheva, E. Landau, M. Shtutman, M. Cohen, K. Arnold, E. Feinstein, B. Geiger, and A. Bershadsky p120 catenin regulates lamellipodial dynamics and cell adhesion in cooperation with cortactin PNAS, June 26, 2007; 104(26): 10882 - 10887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Furmaniak-Kazmierczak, S. W. Crawley, R. L. Carter, D. H. Maurice, and G. P. Cote Formation of Extracellular Matrix-Digesting Invadopodia by Primary Aortic Smooth Muscle Cells Circ. Res., May 11, 2007; 100(9): 1328 - 1336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Morita, T. Mayanagi, T. Yoshio, and K. Sobue Changes in the Balance between Caldesmon Regulated by p21-activated Kinases and the Arp2/3 Complex Govern Podosome Formation J. Biol. Chem., March 16, 2007; 282(11): 8454 - 8463. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, J. M. Urban, M. L. Cayer, H. K. Plummer III, and C. A. Heckman Actin-based features negatively regulated by protein kinase C-{epsilon} Am J Physiol Cell Physiol, November 1, 2006; 291(5): C1002 - C1013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. I. Cosen-Binker and A. Kapus Cortactin: The Gray Eminence of the Cytoskeleton. Physiology, October 1, 2006; 21(5): 352 - 361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. F. G. van Helden, D. J. E. B. Krooshoop, K. C. M. Broers, R. A. P. Raymakers, C. G. Figdor, and F. N. van Leeuwen A Critical Role for Prostaglandin E2 in Podosome Dissolution and Induction of High-Speed Migration during Dendritic Cell Maturation J. Immunol., August 1, 2006; 177(3): 1567 - 1574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Collin, P. Tracqui, A. Stephanou, Y. Usson, J. Clement-Lacroix, and E. Planus Spatiotemporal dynamics of actin-rich adhesion microdomains: influence of substrate flexibility. J. Cell Sci., May 1, 2006; 119(Pt 9): 1914 - 1925. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Eves, B. A. Webb, S. Zhou, and A. S. Mak Caldesmon is an integral component of podosomes in smooth muscle cells J. Cell Sci., May 1, 2006; 119(9): 1691 - 1702. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Lagana, J. G. Goetz, P. Cheung, A. Raz, J. W. Dennis, and I. R. Nabi Galectin Binding to Mgat5-Modified N-Glycans Regulates Fibronectin Matrix Remodeling in Tumor Cells Mol. Cell. Biol., April 15, 2006; 26(8): 3181 - 3193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zhou, B. A. Webb, R. Eves, and A. S. Mak Effects of tyrosine phosphorylation of cortactin on podosome formation in A7r5 vascular smooth muscle cells Am J Physiol Cell Physiol, February 1, 2006; 290(2): C463 - C471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Barberis, A. Casazza, R. Sordella, S. Corso, S. Artigiani, J. Settleman, P. M. Comoglio, and L. Tamagnone p190 Rho-GTPase activating protein associates with plexins and it is required for semaphorin signalling J. Cell Sci., October 15, 2005; 118(20): 4689 - 4700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Webb, R. Eves, S. W. Crawley, S. Zhou, G. P. Cote, and A. S. Mak PAK1 induces podosome formation in A7r5 vascular smooth muscle cells in a PAK-interacting exchange factor-dependent manner Am J Physiol Cell Physiol, October 1, 2005; 289(4): C898 - C907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Destaing, F. Saltel, B. Gilquin, A. Chabadel, S. Khochbin, S. Ory, and P. Jurdic A novel Rho-mDia2-HDAC6 pathway controls podosome patterning through microtubule acetylation in osteoclasts J. Cell Sci., July 1, 2005; 118(13): 2901 - 2911. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Burgstaller and M. Gimona Podosome-mediated matrix resorption and cell motility in vascular smooth muscle cells Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H3001 - H3005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Gangopadhyay, N. Takizawa, C. Gallant, A. L. Barber, H.-D. Je, T. C. Smith, E. J. Luna, and K. G. Morgan Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle J. Cell Sci., October 1, 2004; 117(21): 5043 - 5057. [Abstract] [Full Text] [PDF]
|
|
