QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online December 15, 2003
doi: 10.1242/10.1242/jcs.00856

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Levy, E. Articles by Bendayan, M. Search for Related Content PubMed PubMed Citation Articles by Levy, E. Articles by Bendayan, M.

Ontogeny, immunolocalisation, distribution and function of SR-BI in the human intestine

¹ Department of Nutrition, Hôpital Sainte-Justine and University of Montreal, Montreal QC H3T 1C5, Canada
² Group on the Functional Development and Physiopathology of the Digestive Tract, Canadian Institute of Health Research and Department of Cellular Biology, Faculty of Medicine, Université de Sherbrooke, Sherbrooke QC J1H 5N4, Canada
³ Department of Biochemistry, Hôpital Sainte-Justine and University of Montreal, Montreal QC H3T 1C5, Canada
⁴ Department of Biological Sciences, Université du Québec, Montréal QC H3C 3P8, Canada
⁵ Departments of Pathology and Cell Biology, Hôpital Sainte-Justine and University of Montreal, Montreal QC H3T 1C5, Canada

^* Author for correspondence (e-mail: levye{at}justine.umontreal.ca)

Accepted 5 September 2003

Studies employing human fetal intestine have yielded remarkable information on the role of polarized enterocytes in fat absorption. In this report, we investigated the intestinal expression, spatiotemporal distributions, ontogeny and function of the scavenger receptor, Class B, Type I (SR-BI) that plays a crucial role in cholesterol homeostasis. SR-BI was detected as early as week 14 of gestation in all gut segments and was almost entirely confined to the absorptive epithelial cells. By using immunofluorescence staining, the distribution of SR-BI rarely appeared as a gradient, increasing from the developing crypt to the tip of the villus. Western blot showed high levels of immunodetectable SR-BI in the duodenum, which progressively decreased toward the distal colon. The high-resolution immunogold technique revealed labelling mainly over microvilli of the enterocyte. SR-BI was not associated with caveolin-1 and was not detectable in caveolae. In order to define the role of SR-BI in intestinal cholesterol absorption, Caco-2 cells were transfected with a constitutive expression vector (pZeoSV) containing human SR-BI cDNA inserted in an antisense orientation. As noted by immunoblotting and Protein A-gold techniques, stable transformants contained 40, 60 and 80% the SR-BI level of control Caco-2 cells and exhibited a proportional drop in free cholesterol uptake without altering the capture of phospholipids or cholesteryl ester. Confirmation of these data was obtained in intestinal organ culture where SR-BI antibodies lowered cholesterol uptake. These observations suggest that the human intestine possesses a developmental and regional SR-BI pattern of distribution, and extends our knowledge in SR-BI-mediated cholesterol transport.

Key words: SR-BI, Enterocyte, Cholesterol transport, Caveolin-1, Malabsorption, Atherosclerosis

This article has been cited by other articles:

				A. Montoudis, E. Seidman, F. Boudreau, J.-F. Beaulieu, D. Menard, M. Elchebly, G. Mailhot, A.-T. Sane, M. Lambert, E. Delvin, et al. Intestinal fatty acid binding protein regulates mitochondrion {beta}-oxidation and cholesterol uptake J. Lipid Res., May 1, 2008; 49(5): 961 - 972. [Abstract] [Full Text] [PDF]

				E. Levy, K. Trudel, M. Bendayan, E. Seidman, E. Delvin, M. Elchebly, J.-C. Lavoie, L.-P. Precourt, D. Amre, and D. Sinnett Biological role, protein expression, subcellular localization, and oxidative stress response of paraoxonase 2 in the intestine of humans and rats Am J Physiol Gastrointest Liver Physiol, December 1, 2007; 293(6): G1252 - G1261. [Abstract] [Full Text] [PDF]

				T. Wang, Y. Jiao, and C. Montell Dissection of the pathway required for generation of vitamin A and for Drosophila phototransduction J. Cell Biol., April 23, 2007; 177(2): 305 - 316. [Abstract] [Full Text] [PDF]

				A. T. Sane, D. Sinnett, E. Delvin, M. Bendayan, V. Marcil, D. Menard, J.-F. Beaulieu, and E. Levy Localization and role of NPC1L1 in cholesterol absorption in human intestine J. Lipid Res., October 1, 2006; 47(10): 2112 - 2120. [Abstract] [Full Text] [PDF]

				Y. Jiang, P. Oliver, K. E. Davies, and N. Platt Identification and Characterization of Murine SCARA5, a Novel Class A Scavenger Receptor That Is Expressed by Populations of Epithelial Cells J. Biol. Chem., April 28, 2006; 281(17): 11834 - 11845. [Abstract] [Full Text] [PDF]

				C. J. Harder, G. Vassiliou, H. M. McBride, and R. McPherson Hepatic SR-BI-mediated cholesteryl ester selective uptake occurs with unaltered efficiency in the absence of cellular energy J. Lipid Res., March 1, 2006; 47(3): 492 - 503. [Abstract] [Full Text] [PDF]

				E. Reboul, A. Klein, F. Bietrix, B. Gleize, C. Malezet-Desmoulins, M. Schneider, A. Margotat, L. Lagrost, X. Collet, and P. Borel Scavenger Receptor Class B Type I (SR-BI) Is Involved in Vitamin E Transport across the Enterocyte J. Biol. Chem., February 24, 2006; 281(8): 4739 - 4745. [Abstract] [Full Text] [PDF]

				R. E. Temel, R. G. Lee, K. L. Kelley, M. A. Davis, R. Shah, J. K. Sawyer, M. D. Wilson, and L. L. Rudel Intestinal cholesterol absorption is substantially reduced in mice deficient in both ABCA1 and ACAT2 J. Lipid Res., November 1, 2005; 46(11): 2423 - 2431. [Abstract] [Full Text] [PDF]