|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online 2 December 2003
doi: 10.1242/jcs.00833
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Article |
1 Institut für Biochemie,, Universitätsklinikum RWTH Aachen, Pauwelsstraße 30, 52057 Aachen, Germany
2 Institut für Pharmakologie und Toxikologie, Universitätsklinikum RWTH Aachen, Pauwelsstraße 30, 52057 Aachen, Germany
* Author for correspondence (e-mail: mueller-newen{at}rwth-aachen.de)
Accepted 13 August 2003
Signal transducer and activator of transcription 3 (STAT3) is a transcription factor that is involved in a variety of biological functions. It is essential for the signal transduction of interleukin-6 (IL-6) and related cytokines. In response to IL-6 stimulation STAT3 becomes phosphorylated and translocates into the nucleus where it binds to enhancer sequences of target genes. We found that activated STAT3 is enriched in dot-like structures within the nucleus, which we termed STAT3 nuclear bodies. To examine the dynamics of STAT3 nuclear body formation, a fusion protein of STAT3 and yellow fluorescent protein (YFP) was constructed. Studies in living cells have shown that the appearance of STAT3 nuclear bodies is transient, correlating with the timecourse of tyrosine-phosphorylation of STAT3. Furthermore, we show by fluorescence recovery after photobleaching (FRAP) analysis that STAT3 within nuclear bodies consists of a highly mobile and an immobile fraction. Colocalization studies provided evidence that these bodies are accompanied with CREB binding protein (CBP) and acetylated histone H4, which are markers for transcriptionally active chromatin. Moreover, STAT3 nuclear bodies in HepG2 cells are not colocalized with promyelocytic leukemia oncoprotein (PML)-containing bodies; neither is a sumoylation of activated STAT3 detectable. Taken together, our data suggest that STAT3 nuclear bodies are either directly involved in active gene transcription or they serve as reservoirs of activated STAT3.
Key words: STAT3, Nuclear bodies, FRAP, PML, CBP, Acetylated histones
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  R. Muromoto, Y. Sekine, S. Imoto, O. Ikeda, T. Okayama, N. Sato, and T. Matsuda BART is essential for nuclear retention of STAT3 Int. Immunol., March 1, 2008; 20(3): 395 - 403. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Huang, J. Qiu, S. Dong, M. S. Redell, V. Poli, M. A. Mancini, and D. J. Tweardy Stat3 Isoforms, {alpha} and , Demonstrate Distinct Intracellular Dynamics with Prolonged Nuclear Retention of Stat3 Mapping to Its Unique C-terminal End J. Biol. Chem., November 30, 2007; 282(48): 34958 - 34967. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Herrmann, M. Vogt, M. Monnigmann, T. Clahsen, U. Sommer, S. Haan, V. Poli, P. C. Heinrich, and G. Muller-Newen Nucleocytoplasmic shuttling of persistently activated STAT3 J. Cell Sci., September 15, 2007; 120(18): 3249 - 3261. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Yamashina, H. Yamamoto, K. Chayama, K. Nakajima, and A. Kikuchi Suppression of STAT3 Activity by Duplin, Which Is a Negative Regulator of the Wnt Signal J. Biochem., February 1, 2006; 139(2): 305 - 314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Pranada, S. Metz, A. Herrmann, P. C. Heinrich, and G. Muller-Newen Real Time Analysis of STAT3 Nucleocytoplasmic Shuttling J. Biol. Chem., April 9, 2004; 279(15): 15114 - 15123. [Abstract] [Full Text] [PDF]
|
|
