|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online 25 August 2004
doi: 10.1242/jcs.01337
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Article |
1 Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
2 Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmalaan 121, 1066 CX Amsterdam, The Netherlands
3 Department of Rheumatology, Thurston Arthritis Research Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
* Author for correspondence (e-mail: christin{at}niob.knaw.nl)
Accepted 28 May 2004
Signaling by bone morphogenetic proteins is essential for a wide variety of developmental processes. Receptor-regulated Smad proteins, Smads 1 and 5, are intracellular mediators of bone morphogenetic protein signaling. Together with Smad4, these proteins translocate to the nucleus and modulate transcription by binding to specific sequences on the promoters of target genes. We sought to map transcriptional Smad1/5 activity in development by generating embryonic stem cell lines carrying a Smad1/5-specific response element derived from the Id1 promoter coupled to ß-galactosidase or luciferase as reporters. Three independent lines (BRE-lac1, BRE-lac2 and BRE-luc) have shown the existence of an autocrine bone morphogenetic protein signaling pathway in mouse embryonic stem cells. Reporter activity was detected in chimeric embryos, suggesting sensitivity to physiological concentrations of bone morphogenetic protein. Reporter activity in embryos from transgenic mouse lines was detected in tissues where an essential role for active bone morphogenetic protein signaling via Smads 1 or 5 had been previously established. We have thus generated, for the first time, an in vivo readout for studying the role of Smad1/5-mediated transcriptional activity in development.
Key words: BMP responsive element, Smad1/5, Reporter mice, Embryonic stem cells
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  J. West, J. Harral, K. Lane, Y. Deng, B. Ickes, D. Crona, S. Albu, D. Stewart, and K. Fagan Mice expressing BMPR2R899X transgene in smooth muscle develop pulmonary vascular lesions Am J Physiol Lung Cell Mol Physiol, November 1, 2008; 295(5): L744 - L755. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Craft, L. Xu, D. Romero, C. P. H. Vary, and R. C. Bergan Genistein Induces Phenotypic Reversion of Endoglin Deficiency in Human Prostate Cancer Cells Mol. Pharmacol., January 1, 2008; 73(1): 235 - 242. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. LeClair, T. Durmus, Q. Wang, P. Pyagay, A. Terzic, and V. Lindner Cthrc1 Is a Novel Inhibitor of Transforming Growth Factor-{beta} Signaling and Neointimal Lesion Formation Circ. Res., March 30, 2007; 100(6): 826 - 833. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ogawa, A. Saito, H. Matsui, H. Suzuki, S. Ohtsuka, D. Shimosato, Y. Morishita, T. Watabe, H. Niwa, and K. Miyazono Activin-Nodal signaling is involved in propagation of mouse embryonic stem cells J. Cell Sci., January 1, 2007; 120(1): 55 - 65. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Singbrant, J. L. Moody, U. Blank, G. Karlsson, L. Umans, A. Zwijsen, and S. Karlsson Smad5 is dispensable for adult murine hematopoiesis Blood, December 1, 2006; 108(12): 3707 - 3712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Mazerbourg and A. J.W. Hsueh Genomic analyses facilitate identification of receptors and signalling pathways for growth differentiation factor 9 and related orphan bone morphogenetic protein/growth differentiation factor ligands Hum. Reprod. Update, July 1, 2006; 12(4): 373 - 383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L J Spicer, P Y Aad, D Allen, S Mazerbourg, and A J Hsueh Growth differentiation factor-9 has divergent effects on proliferation and steroidogenesis of bovine granulosa cells. J. Endocrinol., May 1, 2006; 189(2): 329 - 339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Yang, C.-L. Cai, L. Lin, Y. Qyang, C. Chung, R. M. Monteiro, C. L. Mummery, G. I. Fishman, A. Cogen, and S. Evans Isl1Cre reveals a common Bmp pathway in heart and limb development. Development, April 1, 2006; 133(8): 1575 - 1585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Mazerbourg, K. Sangkuhl, C.-W. Luo, S. Sudo, C. Klein, and A. J. W. Hsueh Identification of Receptors and Signaling Pathways for Orphan Bone Morphogenetic Protein/Growth Differentiation Factor Ligands Based on Genomic Analyses J. Biol. Chem., September 16, 2005; 280(37): 32122 - 32132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Watt Best Paper Award 2004 J. Cell Sci., August 1, 2005; 118(15): 3221 - 3221. [Full Text] [PDF]
|
|
|
|
 |
|
 A. H. Lin, J. Luo, L. H. Mondshein, P. ten Dijke, D. Vivien, C. H. Contag, and T. Wyss-Coray Global Analysis of Smad2/3-Dependent TGF-{beta} Signaling in Living Mice Reveals Prominent Tissue-Specific Responses to Injury J. Immunol., July 1, 2005; 175(1): 547 - 554. [Abstract] [Full Text] [PDF]
|
|
