|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online 31 August 2004
doi: 10.1242/jcs.01362
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Article |
Germ Cell Development, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37070 Göttingen, Germany
* Author for correspondence (e-mail: eraz{at}gwdg.de)
Accepted 15 June 2004
During their migration, zebrafish primordial germ cells (PGCs) rely on directional cues provided by the chemokine SDF-1a, whose receptor is CXCR4b. The molecular mechanisms whereby CXCR4b activation is interpreted intracellularly into directional migration are not known. Here we investigate the role of two important biochemical pathways - G-protein-dependent and phosphoinositide 3-kinase (PI3K)-dependent signaling - in directing PGC migration in zebrafish. We show that G proteins of the Gi family are essential for directional migration but not for PGC motility. Inhibition of PI3K signaling in PGCs slows down their migration and leads to abnormal cell morphology as well as to reduced stability of filopodia. Invariably, during directed PGC migration, the distribution of the products of PI3K activity - phosphatidylinositol (3,4,5)-trisphosphate [PtdIns(3,4,5)P3] and/or phosphatidylinositol (3,4)bisphosphate [PtdIns(3,4)P2] - is not polarized, and reducing the level of these 3-phosphoinositides does not affect the ability of PGCs to migrate directionally. We therefore conclude that Gi-dependent signaling is essential for directional migration, whereas the PI3K pathway is important for the actual motility of PGCs.
Key words: Danio rerio, PGC, Chemotaxis, Cell migration, PH domain, G protein, PI3K, SDF-1, CXCR4, Chemokine
Related articles in JCS:
This article has been cited by other articles:
|
|
 |
|
  T. Mizoguchi, H. Verkade, J. K. Heath, A. Kuroiwa, and Y. Kikuchi Sdf1/Cxcr4 signaling controls the dorsal migration of endodermal cells during zebrafish gastrulation Development, August 1, 2008; 135(15): 2521 - 2529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Svetic, G. E. Hollway, S. Elworthy, T. R. Chipperfield, C. Davison, R. J. Adams, J. S. Eisen, P. W. Ingham, P. D. Currie, and R. N. Kelsh Sdf1a patterns zebrafish melanophores and links the somite and melanophore pattern defects in choker mutants Development, March 1, 2007; 134(5): 1011 - 1022. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Yoshida and T. Soldati Dissection of amoeboid movement into two mechanically distinct modes J. Cell Sci., September 15, 2006; 119(18): 3833 - 3844. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Turnpenny, C. M. Spalluto, R. M. Perrett, M. O'Shea, K. P. Hanley, I. T. Cameron, D. I. Wilson, and N. A. Hanley Evaluating Human Embryonic Germ Cells: Concord and Conflict as Pluripotent Stem Cells Stem Cells, February 1, 2006; 24(2): 212 - 220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. I. Cha, S.-H. Kim, D. Sepich, F. G. Buchanan, L. Solnica-Krezel, and R. N. DuBois Cyclooxygenase-1-derived PGE2 promotes cell motility via the G-protein-coupled EP4 receptor during vertebrate gastrulation Genes & Dev., January 1, 2006; 20(1): 77 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Blaser, S. Eisenbeiss, M. Neumann, M. Reichman-Fried, B. Thisse, C. Thisse, and E. Raz Transition from non-motile behaviour to directed migration during early PGC development in zebrafish J. Cell Sci., September 1, 2005; 118(17): 4027 - 4038. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Riol-Blanco, N. Sanchez-Sanchez, A. Torres, A. Tejedor, S. Narumiya, A. L. Corbi, P. Sanchez-Mateos, and J. L. Rodriguez-Fernandez The Chemokine Receptor CCR7 Activates in Dendritic Cells Two Signaling Modules That Independently Regulate Chemotaxis and Migratory Speed J. Immunol., April 1, 2005; 174(7): 4070 - 4080. [Abstract] [Full Text] [PDF]
|
|
