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First published online 31 August 2004
doi: 10.1242/jcs.01371

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Light-dependent subcellular translocation of G_q in Drosophila photoreceptors is facilitated by the photoreceptor-specific myosin III NINAC

Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA

^* Author for correspondence (e-mail: tsunoda{at}bu.edu)

Accepted 21 June 2004

We examine the light-dependent subcellular translocation of the visual G_q protein between the signaling compartment, the rhabdomere and the cell body in Drosophila photoreceptors. We characterize the translocation of G_q and provide the first evidence implicating the involvement of the photoreceptor-specific myosin III NINAC in G_q transport. Translocation of G_q from the rhabdomere to the cell body is rapid, taking less than 5 minutes. Higher light intensities increased the quantity of G_q translocated out of the rhabdomeres from 20% to 75%, consistent with a mechanism for light adaptation. We demonstrate that translocation of G_q requires rhodopsin, but none of the known downstream phototransduction components, suggesting that the signaling pathway triggering translocation occurs upstream of G_q. Finally, we show that ninaC mutants display a significantly reduced rate of G_q transport from the cell body to the rhabdomere, suggesting that NINAC might function as a light-dependent plus-end motor involved in the transport of G_q.

Key words: Light-dependent, Translocation, Drosophila, G-protein, NINAC, Myosin

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