spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 31 August 2004
doi: 10.1242/jcs.01360

Journal of Cell Science 117, 4807-4818 (2004)
Published by The Company of Biologists 2004
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.01360v1
117/20/4807    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JCS
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Næsted, H.
Right arrow Articles by Mundy, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Næsted, H.
Right arrow Articles by Mundy, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Arabidopsis VARIEGATED 3 encodes a chloroplast-targeted, zinc-finger protein required for chloroplast and palisade cell development

Henrik Næsted1, Agnethe Holm1, Tom Jenkins1, H. Bjørn Nielsen2, Cassandra A. Harris3, Michael H. Beale3, Mathias Andersen4, Alexandra Mant5, Henrik Scheller5, Bilal Camara6, Ole Mattsson1 and John Mundy1,*

1 Institute of Molecular Biology, Copenhagen University, Øster Farimagsgade 2A, 1353 Copenhagen K, Denmark
2 BioCentrum, Technical University of Denmark, Building 208, 2800 Lyngby, Denmark
3 Long Ashton Research Station, University of Bristol, Weston Road, Bristol, BS41 9AF, UK
4 Research Center Foulum, PO Box 50, 8839 Tjele, Denmark
5 Institute of Plant Biology, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, 1871 Frederiksberg, Denmark
6 CNRS, Institut de Biologie Moleculaire, Rue de General Zimmer 12, 67084 Strasbourg, France

* Author for correspondence (e-mail: mundy{at}biobase.dk)

Accepted 14 June 2004

The stable, recessive Arabidopsis variegated 3 (var3) mutant exhibits a variegated phenotype due to somatic areas lacking or containing developmentally retarded chloroplasts and greatly reduced numbers of palisade cells. The VAR3 gene, isolated by transposon tagging, encodes the 85.9 kDa VAR3 protein containing novel repeats and zinc fingers described as protein interaction domains. VAR3 interacts specifically in yeast and in vitro with NCED4, a putative polyene chain or carotenoid dioxygenase, and both VAR3 and NCED4 accumulate in the chloroplast stroma. Metabolic profiling demonstrates that pigment profiles are qualitatively similar in wild type and var3, although var3 accumulates lower levels of chlorophylls and carotenoids. These results indicate that VAR3 is a part of a protein complex required for normal chloroplast and palisade cell development.

Key words: VAR3, NCED4, Transposon tagging, Chloroplast development, Stromal subcellular localization


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related articles in JCS:

Lightening up leafy borders

JCS 2004 117: 2004. [Full Text]  



This article has been cited by other articles:


Home page
 Plant Physiol.Home page
G. Holzl, S. Witt, N. Gaude, M. Melzer, M. A. Schottler, and P. Dormann
The Role of Diglycosyl Lipids in Photosynthesis and Membrane Lipid Homeostasis in Arabidopsis
Plant Physiology, July 1, 2009; 150(3): 1147 - 1159.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F.-C. Huang, P. Molnar, and W. Schwab
Cloning and functional characterization of carotenoid cleavage dioxygenase 4 genes
J. Exp. Bot., May 12, 2009; (2009) erp137v1.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Rubio, J. L. Rambla, M. Santaella, M. D. Gomez, D. Orzaez, A. Granell, and L. Gomez-Gomez
Cytosolic and Plastoglobule-targeted Carotenoid Dioxygenases from Crocus sativus Are Both Involved in {beta}-Ionone Release
J. Biol. Chem., September 5, 2008; 283(36): 24816 - 24825.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
A. Prado-Cabrero, D. Scherzinger, J. Avalos, and S. Al-Babili
Retinal Biosynthesis in Fungi: Characterization of the Carotenoid Oxygenase CarX from Fusarium fujikuroi
Eukaryot. Cell, April 1, 2007; 6(4): 650 - 657.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. J. Ytterberg, J.-B. Peltier, and K. J. van Wijk
Protein Profiling of Plastoglobules in Chloroplasts and Chromoplasts. A Surprising Site for Differential Accumulation of Metabolic Enzymes
Plant Physiology, March 1, 2006; 140(3): 984 - 997.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Raynaud, C. Perennes, C. Reuzeau, O. Catrice, S. Brown, and C. Bergounioux
Cell and plastid division are coordinated through the prereplication factor AtCDT1
PNAS, June 7, 2005; 102(23): 8216 - 8221.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004