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First published online October 27, 2004
doi: 10.1242/10.1242/jcs.01473

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Fkh2p and Sep1p regulate mitotic gene transcription in fission yeast

Vicky Buck^1,*, Szu Shien Ng^2,*, Ana Belen Ruiz-Garcia^1,*,, Kyriaki Papadopoulou², Saeeda Bhatti², Jane M. Samuel¹, Mark Anderson², Jonathan B. A. Millar^1, and Christopher J. McInerny^2,

¹ Division of Yeast Genetics, National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, UK
² Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UK

Authors for correspondence (e-mail: jmillar{at}nimr.mrc.ac.uk; c.mcinerny{at}bio.gla.ac.uk)

Accepted 30 July 2004

In the fission yeast Schizosaccharomyces pombe, several genes including cdc15⁺, spo12⁺, fin1⁺, slp1⁺, ace2⁺ and plo1⁺ are periodically expressed during M phase. The products of these genes control various aspects of cell cycle progression including sister chromatid separation, septation and cytokinesis. We demonstrate that periodic expression of these genes is regulated by a common promoter sequence element, named a PCB. In a genetic screen for cell cycle regulators we have identified a novel forkhead transcription factor, Fkh2p, which is periodically phosphorylated in M phase. We show that Fhk2p and another forkhead transcription factor, Sep1p, are necessary for PCB-driven M-phase-specific transcription. In a previous report we identified a complex by electrophoretic mobility shift assay, which we termed PBF, that binds to a 150 bp region of the cdc15⁺ promoter that contains the PCB element. We have identified Mbx1p, a novel MADS box protein, as a component of PBF. However, although Mbx1p is periodically phosphorylated in M phase, Mbx1p is not required for periodic gene transcription in M phase. Moreover, although PBF is absent in strains bearing a C-terminal epitope tag on Fkh2p, simultaneous deletion of fkh2⁺ and sep1⁺ does not abolish PBF binding activity. This suggests that Mbx1p binds to gene promoters, but is not required for transcriptional activation. Together these results suggest that the activation of the Fkh2p and Sep1p forkhead transcription factors triggers mitotic gene transcription in fission yeast.

Key words: Fission yeast, Cell cycle, Transcription, Forkhead, MADS box

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