|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online November 10, 2004
doi: 10.1242/10.1242/jcs.01502
Research Article |
1 Department of Cell Biology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
2 Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
3 Department of Cancer Biology, The University of Texas-MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
Authors for correspondence (e-mail: jeffrey.nickerson{at}umassmed.edu; anthony.imbalzano{at}umassmed.edu)
Accepted 19 August 2004
The SWI/SNF enzymes belong to a family of ATP-dependent chromatin remodeling enzymes that have been functionally implicated in gene regulation, development, differentiation and oncogenesis. BRG1, the catalytic core subunit of some of the SWI/SNF enzymes, can interact with known tumor suppressor proteins and can act as a tumor suppressor itself. We report that cells that inducibly express ATPase-deficient versions of BRG1 increase in cell volume, area of attachment and nuclear size upon expression of the mutant BRG1 protein. Examination of focal adhesions reveals qualitative changes in paxillin distribution but no difference in the actin cytoskeletal structure. Increases in cell size and shape correlate with over-expression of two integrins and the urokinase-type plasminogen activator receptor (uPAR), which is also involved in cell adhesion and is often over-expressed in metastatic cancer cells. These findings demonstrate that gene expression pathways affected by chromatin remodeling enzymes can regulate the physical dimensions of mammalian cell morphology.
Key words: Cell size and shape, Nuclear size, SWI/SNF, BRG1, Chromatin remodeling
This article has been cited by other articles:
|
|
 |
|
  J. Caramel, F. Quignon, and O. Delattre RhoA-Dependent Regulation of Cell Migration by the Tumor Suppressor hSNF5/INI1 Cancer Res., August 1, 2008; 68(15): 6154 - 6161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Mandel and I. Gozes Activity-dependent Neuroprotective Protein Constitutes a Novel Element in the SWI/SNF Chromatin Remodeling Complex J. Biol. Chem., November 23, 2007; 282(47): 34448 - 34456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Guidi, R. Mudhasani, K. Hoover, A. Koff, I. Leav, A. N. Imbalzano, and S. N. Jones Functional Interaction of the Retinoblastoma and Ini1/Snf5 Tumor Suppressors in Cell Growth and Pituitary Tumorigenesis Cancer Res., August 15, 2006; 66(16): 8076 - 8082. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Coisy-Quivy, O. Disson, V. Roure, C. Muchardt, J.-M. Blanchard, and J.-C. Dantonel Role for brm in cell growth control. Cancer Res., May 15, 2006; 66(10): 5069 - 5076. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chi, S. M. Khersonsky, Y.-T. Chang, and V. L. Schuster Identification of a New Class of Prostaglandin Transporter Inhibitors and Characterization of Their Biological Effects on Prostaglandin E2 Transport J. Pharmacol. Exp. Ther., March 1, 2006; 316(3): 1346 - 1350. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. R. Ramirez-Carrozzi, A. A. Nazarian, C. C. Li, S. L. Gore, R. Sridharan, A. N. Imbalzano, and S. T. Smale Selective and antagonistic functions of SWI/SNF and Mi-2beta nucleosome remodeling complexes during an inflammatory response Genes & Dev., February 1, 2006; 20(3): 282 - 296. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Simpson-Holley, R. C. Colgrove, G. Nalepa, J. W. Harper, and D. M. Knipe Identification and Functional Evaluation of Cellular and Viral Factors Involved in the Alteration of Nuclear Architecture during Herpes Simplex Virus 1 Infection J. Virol., October 15, 2005; 79(20): 12840 - 12851. [Abstract] [Full Text] [PDF]
|
|
