QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 16 November 2004
doi: 10.1242/jcs.01523

This Article Figures Only Full Text Full Text (PDF) All Versions of this Article: jcs.01523v1 117/25/6043    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhao, X. Articles by Müller, B. Search for Related Content PubMed PubMed Citation Articles by Zhao, X. Articles by Müller, B.

The human histone gene expression regulator HBP/SLBP is required for histone and DNA synthesis, cell cycle progression and cell proliferation in mitotic cells

School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK

^* Author for correspondence (e-mail: b.mueller{at}abdn.ac.uk)

Accepted 2 September 2004

Histone proteins are essential for chromatin formation, and histone gene expression is coupled to DNA synthesis. In metazoans, the histone RNA binding protein HBP/SLBP is involved in post-transcriptional control of histone gene expression. In vitro assays have demonstrated that human HBP/SLBP is involved in histone mRNA 3' end formation and translation. We have inhibited human HBP/SLBP expression by RNA interference to determine its function during the mitotic cell cycle. Inhibition of HBP/SLBP expression resulted in the inhibition of histone gene expression and DNA synthesis, the inhibition of cell cycle progression in S phase and the inhibition of cell proliferation. These findings indicate that human HBP/SLBP is essential for the coordinate synthesis of DNA and histone proteins and is required for progression through the cell division cycle.

Key words: Cell division cycle, Histone gene expression, DNA synthesis

This article has been cited by other articles:

				N. G. Cakmakci, R. S. Lerner, E. J. Wagner, L. Zheng, and W. F. Marzluff SLIP1, a Factor Required for Activation of Histone mRNA Translation by the Stem-Loop Binding Protein Mol. Cell. Biol., February 1, 2008; 28(3): 1182 - 1194. [Abstract] [Full Text] [PDF]

				B. Muller, J. Blackburn, C. Feijoo, X. Zhao, and C. Smythe DNA-activated protein kinase functions in a newly observed S phase checkpoint that links histone mRNA abundance with DNA replication J. Cell Biol., January 28, 2008; 179(7): 1385 - 1398. [Abstract] [Full Text] [PDF]

				E. J. Wagner and W. F. Marzluff ZFP100, a Component of the Active U7 snRNP Limiting for Histone Pre-mRNA Processing, Is Required for Entry into S Phase. Mol. Cell. Biol., September 1, 2006; 26(17): 6702 - 6712. [Abstract] [Full Text] [PDF]

				R. T. Richardson, O. M. Alekseev, G. Grossman, E. E. Widgren, R. Thresher, E. J. Wagner, K. D. Sullivan, W. F. Marzluff, and M. G. O'Rand Nuclear Autoantigenic Sperm Protein (NASP), a Linker Histone Chaperone That Is Required for Cell Proliferation J. Biol. Chem., July 28, 2006; 281(30): 21526 - 21534. [Abstract] [Full Text] [PDF]

				B. GORGONI, S. ANDREWS, A. SCHALLER, D. SCHUMPERLI, N. K. GRAY, and B. MULLER The stem-loop binding protein stimulates histone translation at an early step in the initiation pathway RNA, July 1, 2005; 11(7): 1030 - 1042. [Abstract] [Full Text] [PDF]