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First published online November 24, 2004
doi: 10.1242/10.1242/jcs.01546

Journal of Cell Science 117, 6217-6226 (2004)
Published by The Company of Biologists 2004
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Research Article

Fad24, a mammalian homolog of Noc3p, is a positive regulator in adipocyte differentiation

Kei Tominaga1,2, Yoshikazu Johmura1, Makoto Nishizuka1 and Masayoshi Imagawa1,*

1 Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan
2 Research Division, Nissui Pharmaceutical Limited, 1075-2 Hokunanmoro, Yuki, Ibaraki 307-0036, Japan

* Author for correspondence (e-mail: imagawa{at}phar.nagoya-cu.ac.jp)

Accepted 16 September 2004

Adipocyte differentiation is controlled by complex actions involving gene expression and signal transduction. From metaphase to anaphase, peroxisome proliferator-activated receptor {gamma}, the CCAAT/enhancer-binding protein family and sterol regulatory element-binding protein-1 are known to function as master regulators. However, the mechanism underlying the earliest step, which triggers the initiation of differentiation, remains unknown. In previous reports, we have isolated a number of genes, whose expression increases in the early stage of differentiation in the mouse 3T3-L1 preadipocyte cell line. Here we report the cloning of the full-length cDNA and characterization of an unknown gene isolated previously and named fad24 (factor for adipocyte differentiation 24). Fad24 encodes a protein consisting of 807 amino acids. The deduced amino acid sequence was shown to have a basic leucine zipper motif and a NOC domain. Expression of fad24 was rapidly induced after stimulation with inducers. Furthermore, overexpression of fad24 in NIH-3T3 cells promoted adipogenesis in the presence of a ligand for peroxisome proliferator-activated receptor {gamma}. FAD24 localizes in the nucleus, especially within nuclear speckles. As the nuclear speckle functions as a nascent transcription and pre-mRNA splicing machinery, there is a possibility that FAD24 functions as one of the components for transcription and/or pre-mRNA splicing and positively regulates adipocyte differentiation.

Key words: Adipocyte differentiation, Obesity, Peroxisome proliferator-activated receptor {gamma}, Nuclear speckles, Adipogenesis, 3T3-L1 cells




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© The Company of Biologists Ltd 2004