|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online 23 November 2004
doi: 10.1242/jcs.01564
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Article |
1 Research School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane, Oxford, OX3 0BP, UK
2 Department of Biology, University of Saskatchewan, S7N 5E2, Canada
3 UMR-CNRS 6037, Université de Rouen, 76821 Mont Saint Aignan, France
4 Department of Plant Sciences, University of Oxford, South Parks Road, OX1 3RB, UK
* Author for correspondence (e-mail: chawes{at}brookes.ac.uk)
Accepted 24 September 2004
Rab GTPases are universal key regulators of intracellular secretory trafficking events. In particular, Rab 5 homologues have been implicated in endocytic events and in the vacuolar pathway. In this study, we investigate the location and function of a member of this family, AtRabF2b (Ara7) in tobacco (Nicotiana tabacum) leaf epidermal cells using a live cell imaging approach. Fluorescent-tagged AtRabF2b[wt] localized to the prevacuolar compartment and Golgi apparatus, as determined by coexpression studies with fluorescent markers for these compartments. Mutations that impair AtRabF2b function also alter the subcellular location of the GTPase. In addition, coexpression studies of the protein with the vacuole-targeted aleurain-green fluorescent protein (GFP) and rescue experiments with wild-type AtRabF2b indicate that the dominant-negative mutant of AtRabF2b causes the vacuolar marker to be secreted to the apoplast. Our results indicate a clear role of AtRabF2b in the vacuolar trafficking pathway.
Key words: Rab GTPase, Prevacuolar compartment, Golgi apparatus, Plant secretory pathway
This article has been cited by other articles:
|
|
 |
|
  G. Lycett The role of Rab GTPases in cell wall metabolism J. Exp. Bot., November 1, 2008; 59(15): 4061 - 4074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Yuksel and A. R. Memon Comparative phylogenetic analysis of small GTP-binding genes of model legume plants and assessment of their roles in root nodules J. Exp. Bot., October 9, 2008; (2008) ern223v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Guermonprez, A. Smertenko, M.-T. Crosnier, M. Durandet, N. Vrielynck, P. Guerche, P. J. Hussey, B. Satiat-Jeunemaitre, and S. Bonhomme The POK/AtVPS52 protein localizes to several distinct post-Golgi compartments in sporophytic and gametophytic cells J. Exp. Bot., August 1, 2008; 59(11): 3087 - 3098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Onelli, C. Prescianotto-Baschong, M. Caccianiga, and A. Moscatelli Clathrin-dependent and independent endocytic pathways in tobacco protoplasts revealed by labelling with charged nanogold J. Exp. Bot., August 1, 2008; 59(11): 3051 - 3068. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Held, A. Boulaflous, and F. Brandizzi Advances in Fluorescent Protein-Based Imaging for the Analysis of Plant Endomembranes Plant Physiology, August 1, 2008; 147(4): 1469 - 1481. [Full Text] [PDF]
|
|
|
|
|
|
D. G. Robinson, L. Jiang, and K. Schumacher The Endosomal System of Plants: Charting New and Familiar Territories Plant Physiology, August 1, 2008; 147(4): 1482 - 1492. [Full Text] [PDF]
|
|
|
|
|
|
E. Rojo and J. Denecke What Is Moving in the Secretory Pathway of Plants? Plant Physiology, August 1, 2008; 147(4): 1493 - 1503. [Full Text] [PDF]
|
|
|
|
|
|
E. Nielsen, A. Y. Cheung, and T. Ueda The Regulatory RAB and ARF GTPases for Vesicular Trafficking Plant Physiology, August 1, 2008; 147(4): 1516 - 1526. [Full Text] [PDF]
|
|
|
|
 |
|
 C.-M. Chow, H. Neto, C. Foucart, and I. Moore Rab-A2 and Rab-A3 GTPases Define a trans-Golgi Endosomal Membrane Domain in Arabidopsis That Contributes Substantially to the Cell Plate PLANT CELL, January 1, 2008; 20(1): 101 - 123. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Moscatelli, F. Ciampolini, S. Rodighiero, E. Onelli, M. Cresti, N. Santo, and A. Idilli Distinct endocytic pathways identified in tobacco pollen tubes using charged nanogold J. Cell Sci., November 1, 2007; 120(21): 3804 - 3819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Goh, W. Uchida, S. Arakawa, E. Ito, T. Dainobu, K. Ebine, M. Takeuchi, K. Sato, T. Ueda, and A. Nakano VPS9a, the Common Activator for Two Distinct Types of Rab5 GTPases, Is Essential for the Development of Arabidopsis thaliana PLANT CELL, November 1, 2007; 19(11): 3504 - 3515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Muller, U. Mettbach, D. Menzel, and J. Samaj Molecular Dissection of Endosomal Compartments in Plants Plant Physiology, October 1, 2007; 145(2): 293 - 304. [Full Text] [PDF]
|
|
|
|
|
|
T. J. Haas, M. K. Sliwinski, D. E. Martinez, M. Preuss, K. Ebine, T. Ueda, E. Nielsen, G. Odorizzi, and M. S. Otegui The Arabidopsis AAA ATPase SKD1 Is Involved in Multivesicular Endosome Function and Interacts with Its Positive Regulator LYST-INTERACTING PROTEIN5 PLANT CELL, April 1, 2007; 19(4): 1295 - 1312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Boutte, M.-T. Crosnier, N. Carraro, J. Traas, and B. Satiat-Jeunemaitre The plasma membrane recycling pathway and cell polarity in plants: studies on PIN proteins J. Cell Sci., April 1, 2006; 119(7): 1255 - 1265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Dettmer, A. Hong-Hermesdorf, Y.-D. Stierhof, and K. Schumacher Vacuolar H+-ATPase Activity Is Required for Endocytic and Secretory Trafficking in Arabidopsis PLANT CELL, March 1, 2006; 18(3): 715 - 730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Chatre, F. Brandizzi, A. Hocquellet, C. Hawes, and P. Moreau Sec22 and Memb11 Are v-SNAREs of the Anterograde Endoplasmic Reticulum-Golgi Pathway in Tobacco Leaf Epidermal Cells Plant Physiology, November 1, 2005; 139(3): 1244 - 1254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. A. Sparkes, C. Hawes, and A. Baker AtPEX2 and AtPEX10 Are Targeted to Peroxisomes Independently of Known Endoplasmic Reticulum Trafficking Routes Plant Physiology, October 1, 2005; 139(2): 690 - 700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zheng, L. Camacho, E. Wee, H. Batoko, J. Legen, C. J. Leaver, R. Malho, P. J. Hussey, and I. Moore A Rab-E GTPase Mutant Acts Downstream of the Rab-D Subclass in Biosynthetic Membrane Traffic to the Plasma Membrane in Tobacco Leaf Epidermis PLANT CELL, July 1, 2005; 17(7): 2020 - 2036. [Abstract] [Full Text] [PDF]
|
|
