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First published online 30 November 2004
doi: 10.1242/jcs.01604

Journal of Cell Science 117, 6435-6445 (2004)
Published by The Company of Biologists 2004
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Research Article

Distinct functions of condensin I and II in mitotic chromosome assembly

Toru Hirota1,*, Daniel Gerlich2,*, Birgit Koch1, Jan Ellenberg2 and Jan-Michael Peters1,{ddagger}

1 Research Institute of Molecular Pathology, Dr Bohr-Gasse 7, 1030 Vienna, Austria
2 European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany

{ddagger} Author for correspondence (e-mail: peters{at}imp.univie.ac.at)

Accepted 22 October 2004

Condensin is a protein complex associated with mitotic chromosomes that has been implicated in chromosome condensation. In vertebrates, two types of condensin complexes have recently been identified, called condensin I and II. Here, we show that in mammalian cells condensin II associates with chromatin in prophase, in contrast to condensin I which is cytoplasmic and can thus interact with chromosomes only after nuclear envelope breakdown. RNA interference experiments in conjunction with imaging of live and fixed cells revealed that condensin II is required for chromosome condensation in early prophase, whereas condensin I appears to be dispensable at this stage. By contrast, condensin I is required for the complete dissociation of cohesin from chromosome arms, for chromosome shortening and for normal timing of progression through prometaphase and metaphase, whereas normal condensin II levels are dispensable for these processes. After depletion of both condensin complexes, the onset of chromosome condensation is delayed until the end of prophase, but is then initiated rapidly before nuclear envelope breakdown. These results reveal that condensin II and I associate with chromosomes sequentially and have distinct functions in mitotic chromosome assembly.

Key words: Chromatin, Condensation, Cohesin, Cohesion, Prophase


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