spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 16 December 2003
doi: 10.1242/jcs.00868

Journal of Cell Science 117, 397-405 (2004)
Published by The Company of Biologists 2004
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00868v1
117/3/397    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JCS
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhao, M.
Right arrow Articles by McCaig, C. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhao, M.
Right arrow Articles by McCaig, C. D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Electrical stimulation directly induces pre-angiogenic responses in vascular endothelial cells by signaling through VEGF receptors

Min Zhao*,{ddagger}, Huai Bai*, Entong Wang, John V. Forrester and Colin D. McCaig

Departments of Biomedical Sciences and Ophthalmology, Institute of Medical Sciences, University of Aberdeen, Aberdeen, AB25 2ZD, UK

{ddagger} Author for correspondence (e-mail: m.zhao{at}abdn.ac.uk)

Accepted 12 September 2003

Controlling angiogenesis is crucial. Growth factors and cytokines are key regulators but a full understanding remains elusive. Endogenous electrical potential differences exist within and around the vasculature, both in relation to blood flow and in situations where active angiogenesis occurs, such as wound healing, development and tumor growth. Recent work shows that electrical stimulation induces significant angiogenesis in vivo, through enhanced vascular endothelial growth factor (VEGF) production by muscle cells. We report that applied electric fields (EFs) of small physiological magnitude directly stimulate VEGF production by endothelial cells in culture without the presence of any other cell type. EFs as low as 75-100 mV mm–1 (1.5-2.0 mV across an endothelial cell) directed the reorientation, elongation and migration of endothelial cells in culture. These pre-angiogenic responses required VEGF receptor activation and were mediated through PI3K-Akt and Rho-ROCK signaling pathways, resulting in reorganization of the actin cytoskeleton. This indicates that endogenous EFs might play a role in angiogenesis in vivo by stimulating the VEGF receptor signaling pathway, to induce key pre-angiogenic responses. In addition, it raises the feasibility of using applied EFs to initiate and guide angiogenesis through direct effects on endothelial cells.

Key words: Endothelium, Electrical stimulation, Angiogenesis, Cell alignment, Signal transduction


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related articles in JCS:

Electrical stimulation for endothelia

JCS 2004 117: 301. [Full Text]  



This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
M. J. Sato, H. Kuwayama, W. N. van Egmond, A. L. K. Takayama, H. Takagi, P. J. M. van Haastert, T. Yanagida, and M. Ueda
Switching direction in electric-signal-induced cell migration by cyclic guanosine monophosphate and phosphatidylinositol signaling
PNAS, April 21, 2009; 106(16): 6667 - 6672.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. Lin, F. Baldessari, C. C. Gyenge, T. Sato, R. D. Chambers, J. G. Santiago, and E. C. Butcher
Lymphocyte Electrotaxis In Vitro and In Vivo
J. Immunol., August 15, 2008; 181(4): 2465 - 2471.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
S. Sun, Y. Liu, S. Lipsky, and M. Cho
Physical manipulation of calcium oscillations facilitates osteodifferentiation of human mesenchymal stem cells
FASEB J, May 1, 2007; 21(7): 1472 - 1480.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
L. J. Shanley, P. Walczysko, M. Bain, D. J. MacEwan, and M. Zhao
Influx of extracellular Ca2+ is necessary for electrotaxis in Dictyostelium
J. Cell Sci., November 15, 2006; 119(22): 4741 - 4748.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Pu and M. Zhao
Golgi polarization in a strong electric field
J. Cell Sci., March 15, 2005; 118(6): 1117 - 1128.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Radisic, H. Park, H. Shing, T. Consi, F. J. Schoen, R. Langer, L. E. Freed, and G. Vunjak-Novakovic
From the Cover: Functional assembly of engineered myocardium by electrical stimulation of cardiac myocytes cultured on scaffolds
PNAS, December 28, 2004; 101(52): 18129 - 18134.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
H. Bai, C. D. McCaig, J. V. Forrester, and M. Zhao
DC Electric Fields Induce Distinct Preangiogenic Responses in Microvascular and Macrovascular Cells
Arterioscler. Thromb. Vasc. Biol., July 1, 2004; 24(7): 1234 - 1239.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. E. Mycielska and M. B. A. Djamgoz
Cellular mechanisms of direct-current electric field effects: galvanotaxis and metastatic disease
J. Cell Sci., May 1, 2004; 117(9): 1631 - 1639.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004