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First published online 6 January 2004
doi: 10.1242/jcs.00867

Journal of Cell Science 117, 571-581 (2004)
Published by The Company of Biologists 2004
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Research Article

Murine tenascin-W: a novel mammalian tenascin expressed in kidney and at sites of bone and smooth muscle development

A. Scherberich1, R. P. Tucker1,2, E. Samandari1, M. Brown-Luedi1, D. Martin1 and R. Chiquet-Ehrismann1,*

1 Friedrich Miescher Institute for Biomedical Research, Novartis Research Foundation, Maulbeerstrasse 66, CH-4058 Basel, Switzerland
2 Department of Cell Biology and Human Anatomy, University of California at Davis, Davis, CA 95616, USA

* Author for correspondence (e-mail: Ruth.Chiquet{at}fmi.ch)

Accepted 12 September 2003

We cloned and characterized a novel member of the tenascin family of extracellular matrix proteins - the murine orthologue of zebrafish tenascin-W. Full-length recombinant tenascin-W was expressed and purified from mammalian cell cultures. Rotary shadowing followed by electron microscopy showed that tenascin-W forms hexabrachions. We studied its expression during development and in the adult by immunohistochemistry, in situ hybridization and immunoblotting. Tenascin-W is expressed during palate formation, osteogenesis and smooth muscle development. In the adult, tenascin-W is found in the kidney, cardiac semilunar valves, corneal limbus and periosteum. Tenascin-W and tenascin-C expression overlap in many of these areas. Bone-morphogenic-protein-2 treated C2C12 cells secrete tenascin-W and are able to adhere to and to extend actin-rich processes on a tenascin-W substratum. In vitro, cells bind to tenascin-W in an RGD-dependent manner. This adhesion is increased by transfection of {alpha}8 integrin, which localizes with tenascin-W in the periosteum and kidney.

Key words: Tenascin, Hexabrachion, Expression, Development, Adhesion, Extracellular matrix




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