|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online March 2, 2004
doi: 10.1242/10.1242/jcs.00970
Research Article |
1 Monash Institute of Reproduction and Development, Monash University, 246 Clayton Road, Clayton, Victoria 3168, Australia
2 The Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
* Author for correspondence (e-mail: martin.pera{at}med.monash.edu.au)
Accepted 5 November 2003
Human embryonic stem cells differentiate spontaneously in vitro into a range of cell types, and they frequently give rise to cells with the properties of extra-embryonic endoderm. We show here that endogenous signaling by bone morphogenetic protein-2 controls the differentiation of embryonic stem cells into this lineage. Treatment of embryonic stem cell cultures with the bone morphogenetic protein antagonist noggin blocks this form of differentiation and induces the appearance of a novel cell type that can give rise to neural precursors. These findings indicate that bone morphogenetic protein-2 controls a key early commitment step in human embryonic stem cell differentiation, and show that the conservation of developmental mechanisms at the cellular level can be exploited in this system – in this case, to provide a facile route for the generation of neural precursors from pluripotent cells.
Key words: Human embryonic stem cell, Noggin, Bone morphogenetic protein, Extra-embryonic endoderm, Neural, Differentiation
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
Related articles in JCS:
This article has been cited by other articles:
|
|
 |
|
  G. C. Douglas, C. A. VandeVoort, P. Kumar, T.-C. Chang, and T. G. Golos Trophoblast Stem Cells: Models for Investigating Trophectoderm Differentiation and Placental Development Endocr. Rev., May 1, 2009; 30(3): 228 - 240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wang, Y. Shen, X. Yuan, K. Chen, X. Guo, Y. Chen, Y. Niu, J. Li, R.-H. Xu, X. Yan, et al. Dissecting Signaling Pathways That Govern Self-renewal of Rabbit Embryonic Stem Cells J. Biol. Chem., December 19, 2008; 283(51): 35929 - 35940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Furue, J. Na, J. P. Jackson, T. Okamoto, M. Jones, D. Baker, R.-I. Hata, H. D. Moore, J. D. Sato, and P. W. Andrews Heparin promotes the growth of human embryonic stem cells in a defined serum-free medium PNAS, September 9, 2008; 105(36): 13409 - 13414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Wu, W. Zhang, G. Chen, L. Cheng, J. Liao, N. Jia, Y. Gao, H. Dai, J. Yuan, L. Cheng, et al. Combinatorial Signals of Activin/Nodal and Bone Morphogenic Protein Regulate the Early Lineage Segregation of Human Embryonic Stem Cells J. Biol. Chem., September 5, 2008; 283(36): 24991 - 25002. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. W. Yeo, N. Coufal, S. Aigner, B. Winner, J. A. Scolnick, M. C.N. Marchetto, A. R. Muotri, C. Carson, and F. H. Gage Multiple layers of molecular controls modulate self-renewal and neuronal lineage specification of embryonic stem cells Hum. Mol. Genet., April 15, 2008; 17(R1): R67 - R75. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Gaulden and J. F. Reiter Neur-ons and neur-offs: regulators of neural induction in vertebrate embryos and embryonic stem cells Hum. Mol. Genet., April 15, 2008; 17(R1): R60 - R66. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Zhang, J. Li, Z. Tan, C. Wang, T. Liu, L. Chen, J. Yong, W. Jiang, X. Sun, L. Du, et al. Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells Blood, February 15, 2008; 111(4): 1933 - 1941. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-C. Zhang, X.-J. Li, M Austin Johnson, and M. T Pankratz Human embryonic stem cells for brain repair? Phil Trans R Soc B, January 12, 2008; 363(1489): 87 - 99. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Puceat TGF{beta} in the differentiation of embryonic stem cells Cardiovasc Res, May 1, 2007; 74(2): 256 - 261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kennedy, S. L. D'Souza, M. Lynch-Kattman, S. Schwantz, and G. Keller Development of the hemangioblast defines the onset of hematopoiesis in human ES cell differentiation cultures Blood, April 1, 2007; 109(7): 2679 - 2687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Sneddon, H. H. Zhen, K. Montgomery, M. van de Rijn, A. D. Tward, R. West, H. Gladstone, H. Y. Chang, G. S. Morganroth, A. E. Oro, et al. Bone morphogenetic protein antagonist gremlin 1 is widely expressed by cancer-associated stromal cells and can promote tumor cell proliferation PNAS, October 3, 2006; 103(40): 14842 - 14847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Trounson The Production and Directed Differentiation of Human Embryonic Stem Cells Endocr. Rev., April 1, 2006; 27(2): 208 - 219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Poon, F. Clermont, M. T. Firpo, and R. J. Akhurst TGF{beta} inhibition of yolk-sac-like differentiation of human embryonic stem-cell-derived embryoid bodies illustrates differences between early mouse and human development J. Cell Sci., February 15, 2006; 119(4): 759 - 768. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Levine and A. H. Brivanlou GDF3, a BMP inhibitor, regulates cell fate in stem cells and early embryos Development, January 15, 2006; 133(2): 209 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Keller Embryonic stem cell differentiation: emergence of a new era in biology and medicine Genes & Dev., May 15, 2005; 19(10): 1129 - 1155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Pera and A. O. Trounson Human embryonic stem cells: prospects for development Development, November 15, 2004; 131(22): 5515 - 5525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Trounson Stem cells, plasticity and cancer - uncomfortable bed fellows Development, June 15, 2004; 131(12): 2763 - 2768. [Abstract] [Full Text] [PDF]
|
|
