|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online March 12, 2004
doi: 10.1242/10.1242/jcs.01006
Research Article |
School of Biological Sciences, University of Manchester, 2.205 Stopford Building, Oxford Road, Manchester M13 9PT, UK
* Author for correspondence (e-mail: stephen.taylor{at}man.ac.uk)
Accepted 24 November 2003
During mitosis, the recruitment of spindle-checkpoint-associated proteins to the kinetochore occurs in a defined order. The protein kinase Bub1 localizes to the kinetochore very early during mitosis, followed by Cenp-F, BubR1, Cenp-E and finally Mad2. Using RNA interference, we have investigated whether this order of binding reflects a level of dependency in human somatic cells. Specifically, we show that Bub1 plays a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of Cenp-F, BubR1, Cenp-E and Mad2. In contrast to studies in Xenopus, we also show that BubR1 is not required for kinetochore localization of Bub1. Repression of Bub1 increases the number of cells with lagging chromosomes at metaphase, suggesting that Bub1 plays a role in chromosome congression. However, repression of Bub1 does not appear to compromise spindle checkpoint function either during normal mitosis or in response to spindle damage. This raises the possibility that, in the absence of Bub1, other mechanisms contribute to spindle checkpoint function.
Key words: Kinetochore, Mitosis, Bub1, Aurora B
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
Related articles in JCS:
This article has been cited by other articles:
|
|
 |
|
  J. Kang and H. Yu Kinase Signaling in the Spindle Checkpoint J. Biol. Chem., June 5, 2009; 284(23): 15359 - 15363. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Klebig, D. Korinth, and P. Meraldi Bub1 regulates chromosome segregation in a kinetochore-independent manner J. Cell Biol., June 1, 2009; 185(5): 841 - 858. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Essex, A. Dammermann, L. Lewellyn, K. Oegema, and A. Desai Systematic Analysis in Caenorhabditis elegans Reveals that the Spindle Checkpoint Is Composed of Two Largely Independent Branches Mol. Biol. Cell, February 1, 2009; 20(4): 1252 - 1267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Schliekelman, D. O. Cowley, R. O'Quinn, T. G. Oliver, L. Lu, E.D. Salmon, and T. Van Dyke Impaired Bub1 Function In vivo Compromises Tension-Dependent Checkpoint Function Leading to Aneuploidy and Tumorigenesis Cancer Res., January 1, 2009; 69(1): 45 - 54. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Civril and A. Musacchio Spindly attachments Genes & Dev., September 1, 2008; 22(17): 2302 - 2307. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Umeyama and C. C. Wang Polo-Like Kinase Is Expressed in S/G2/M Phase and Associated with the Flagellum Attachment Zone in both Procyclic and Bloodstream Forms of Trypanosoma brucei Eukaryot. Cell, September 1, 2008; 7(9): 1582 - 1590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Saitoh, Y. Kobayashi, Y. Ogiyama, and K. Takahashi Dual Regulation of Mad2 Localization on Kinetochores by Bub1 and Dam1/DASH that Ensure Proper Spindle Interaction Mol. Biol. Cell, September 1, 2008; 19(9): 3885 - 3897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zhu, F. Wang, F. Yan, P. Y. Yao, J. Du, X. Gao, X. Wang, Q. Wu, T. Ward, J. Li, et al. Septin 7 Interacts with Centromere-associated Protein E and Is Required for Its Kinetochore Localization J. Biol. Chem., July 4, 2008; 283(27): 18916 - 18925. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Emanuele, W. Lan, M. Jwa, S. A. Miller, C. S.M. Chan, and P. T. Stukenberg Aurora B kinase and protein phosphatase 1 have opposing roles in modulating kinetochore assembly J. Cell Biol., April 21, 2008; 181(2): 241 - 254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Logarinho, T. Resende, C. Torres, and H. Bousbaa The Human Spindle Assembly Checkpoint Protein Bub3 Is Required for the Establishment of Efficient Kinetochore-Microtubule Attachments Mol. Biol. Cell, April 1, 2008; 19(4): 1798 - 1813. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Greene, G. Campiani, M. Lawler, D. C. Williams, and D. M. Zisterer BubR1 Is Required for a Sustained Mitotic Spindle Checkpoint Arrest in Human Cancer Cells Treated with Tubulin-Targeting Pyrrolo-1,5-Benzoxazepines Mol. Pharmacol., February 1, 2008; 73(2): 419 - 430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Jeganathan, L. Malureanu, D. J. Baker, S. C. Abraham, and J. M. van Deursen Bub1 mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis J. Cell Biol., October 22, 2007; 179(2): 255 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhang, S. Ahmad, and Y. Mao BubR1 and APC/EB1 cooperate to maintain metaphase chromosome alignment J. Cell Biol., August 27, 2007; 178(5): 773 - 784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Liu, X. Ding, J. Du, X. Cai, Y. Huang, T. Ward, A. Shaw, Y. Yang, R. Hu, C. Jin, et al. Human NUF2 Interacts with Centromere-associated Protein E and Is Essential for a Stable Spindle Microtubule-Kinetochore Attachment J. Biol. Chem., July 20, 2007; 282(29): 21415 - 21424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Niikura, A. Dixit, R. Scott, G. Perkins, and K. Kitagawa BUB1 mediation of caspase-independent mitotic death determines cell fate J. Cell Biol., July 10, 2007; 178(2): 283 - 296. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. V. Gjoerup, J. Wu, D. Chandler-Militello, G. L. Williams, J. Zhao, B. Schaffhausen, P. S. Jat, and T. M. Roberts Surveillance mechanism linking Bub1 loss to the p53 pathway PNAS, May 15, 2007; 104(20): 8334 - 8339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Huang, J. Feng, J. Famulski, J. B. Rattner, S. T. Liu, G. D. Kao, R. Muschel, G. K.T. Chan, and T. J. Yen Tripin/hSgo2 recruits MCAK to the inner centromere to correct defective kinetochore attachments J. Cell Biol., May 7, 2007; 177(3): 413 - 424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Boyarchuk, A. Salic, M. Dasso, and A. Arnaoutov Bub1 is essential for assembly of the functional inner centromere J. Cell Biol., March 26, 2007; 176(7): 919 - 928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Orr, H. Bousbaa, and C. E. Sunkel Mad2-independent Spindle Assembly Checkpoint Activation and Controlled Metaphase-Anaphase Transition in Drosophila S2 Cells Mol. Biol. Cell, March 1, 2007; 18(3): 850 - 863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Qi and H. Yu KEN-Box-dependent Degradation of the Bub1 Spindle Checkpoint Kinase by the Anaphase-promoting Complex/Cyclosome J. Biol. Chem., February 9, 2007; 282(6): 3672 - 3679. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Dikovskaya, D. Schiffmann, I. P. Newton, A. Oakley, K. Kroboth, O. Sansom, T. J. Jamieson, V. Meniel, A. Clarke, and I. S. Nathke Loss of APC induces polyploidy as a result of a combination of defects in mitosis and apoptosis J. Cell Biol., January 16, 2007; 176(2): 183 - 195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Mondal, S. Sengupta, C. K. Panda, S. M. Gollin, W. S. Saunders, and S. Roychoudhury Overexpression of Cdc20 leads to impairment of the spindle assembly checkpoint and aneuploidization in oral cancer Carcinogenesis, January 1, 2007; 28(1): 81 - 92. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-T. Liu, J. B. Rattner, S. A. Jablonski, and T. J. Yen Mapping the assembly pathways that specify formation of the trilaminar kinetochore plates in human cells J. Cell Biol., October 9, 2006; 175(1): 41 - 53. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. V. Holt, M. A. S. Vergnolle, D. Hussein, M. J. Wozniak, V. J. Allan, and S. S. Taylor Silencing Cenp-F weakens centromeric cohesion, prevents chromosome alignment and activates the spindle checkpoint J. Cell Sci., October 15, 2005; 118(20): 4889 - 4900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Morrow, A. Tighe, V. L. Johnson, M. I.F. Scott, C. Ditchfield, and S. S. Taylor Bub1 and aurora B cooperate to maintain BubR1-mediated inhibition of APC/CCdc20 J. Cell Sci., August 15, 2005; 118(16): 3639 - 3652. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Oikawa, M. Okuda, Z. Ma, R. Goorha, H. Tsujimoto, H. Inokuma, and K. Fukasawa Transcriptional Control of BubR1 by p53 and Suppression of Centrosome Amplification by BubR1 Mol. Cell. Biol., May 15, 2005; 25(10): 4046 - 4061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Yang, J. Guo, Q. Chen, C. Ding, J. Du, and X. Zhu Silencing Mitosin Induces Misaligned Chromosomes, Premature Chromosome Decondensation before Anaphase Onset, and Mitotic Cell Death Mol. Cell. Biol., May 15, 2005; 25(10): 4062 - 4074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Dai, S. Sultan, S. S. Taylor, and J. M.G. Higgins The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment Genes & Dev., February 15, 2005; 19(4): 472 - 488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Watrin and V. Legagneux Contribution of hCAP-D2, a Non-SMC Subunit of Condensin I, to Chromosome and Chromosomal Protein Dynamics during Mitosis Mol. Cell. Biol., January 15, 2005; 25(2): 740 - 750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kadura, X. He, V. Vanoosthuyse, K. G. Hardwick, and S. Sazer The A78V Mutation in the Mad3-like Domain of Schizosaccharomyces pombe Bub1p Perturbs Nuclear Accumulation and Kinetochore Targeting of Bub1p, Bub3p, and Mad3p and Spindle Assembly Checkpoint Function Mol. Biol. Cell, January 1, 2005; 16(1): 385 - 395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wang, X. Hu, X. Ding, Z. Dou, Z. Yang, A. W. Shaw, M. Teng, D. W. Cleveland, M. L. Goldberg, L. Niu, et al. Human Zwint-1 Specifies Localization of Zeste White 10 to Kinetochores and Is Essential for Mitotic Checkpoint Signaling J. Biol. Chem., December 24, 2004; 279(52): 54590 - 54598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Vanoosthuyse, R. Valsdottir, J.-P. Javerzat, and K. G. Hardwick Kinetochore Targeting of Fission Yeast Mad and Bub Proteins Is Essential for Spindle Checkpoint Function but Not for All Chromosome Segregation Roles of Bub1p Mol. Cell. Biol., November 15, 2004; 24(22): 9786 - 9801. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Maiato, J. DeLuca, E. D. Salmon, and W. C. Earnshaw The dynamic kinetochore-microtubule interface J. Cell Sci., November 1, 2004; 117(23): 5461 - 5477. [Abstract] [Full Text] [PDF]
|
|
