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First published online 15 December 2004
doi: 10.1242/jcs.01603

Journal of Cell Science 118, 129-136 (2005)
Published by The Company of Biologists 2005
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Research Article

Localisation of human Y-family DNA polymerase {kappa}: relationship to PCNA foci

Tomoo Ogi, Patricia Kannouche* and Alan R. Lehmann{ddagger}

Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton, BN1 9RR, UK

{ddagger} Author for correspondence (e-mail: a.r.lehmann{at}sussex.ac.uk)

Accepted 21 October 2004

DNA polymerases of the Y-family are involved in translesion DNA synthesis past different types of DNA damage. Previous work has shown that DNA polymerases {eta} and {iota} are localised in replication factories during S phase, where they colocalise one-to-one with PCNA. Cells with factories containing these polymerases accumulate after treatment with DNA damaging agents because replication forks are stalled at sites of damage. We now show that DNA polymerase {kappa} (pol{kappa}) has a different localisation pattern. Although, like the other Y-family polymerases, it is exclusively localised in the nucleus, pol{kappa} is found in replication foci in only a small proportion of S-phase cells. It does not colocalise in those foci with proliferating cell nuclear antigen (PCNA) in the majority of cells. This reduced number of cells with pol{kappa} foci, when compared with those containing pol{eta} foci, is observed both in untreated cells and in cells treated with hydroxyurea, UV irradiation or benzo[a]pyrene. The C-terminal 97 amino acids of pol{kappa} are sufficient for this limited localisation into nuclear foci, and include a C2HC zinc finger, bipartite nuclear localisation signal and putative PCNA binding site.

Key words: DNA polymerase, PCNA, Replication foci, Translesion synthesis, UV irradiation




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