QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online December 22, 2004
doi: 10.1242/10.1242/jcs.01602

This Article Figures Only Full Text Full Text (PDF) Supplementary Material Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lopes, C. S. Articles by Sunkel, C. E. Search for Related Content PubMed PubMed Citation Articles by Lopes, C. S. Articles by Sunkel, C. E.

The Drosophila Bub3 protein is required for the mitotic checkpoint and for normal accumulation of cyclins during G2 and early stages of mitosis

¹ Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal
² Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
³ Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Largo do Prof. Abel Salazar n° 2, 4099-003 Porto, Portugal

^* Author for correspondence (e-mail: cesunkel{at}ibmc.up.pt)

Accepted 20 October 2004

During mitosis, a checkpoint mechanism delays metaphase-anaphase transition in the presence of unattached and/or unaligned chromosomes. This delay is achieved through inhibition of the anaphase promoting complex/cyclosome (APC/C) preventing sister chromatid separation and cyclin degradation. In the present study, we show that Bub3 is an essential protein required during normal mitotic progression to prevent premature sister chromatid separation, missegreation and aneuploidy. We also found that Bub3 is required during G2 and early stages of mitosis to promote normal mitotic entry. We show that loss of Bub3 function by mutation or RNAi depletion causes cells to progress slowly through prophase, a delay that appears to result from a failure to accumulate mitotic cyclins A and B. Defective accumulation of mitotic cyclins results from inappropriate APC/C activity, as mutations in the gene encoding the APC/C subunit cdc27 partially rescue this phenotype. Furthermore, analysis of mitotic progression in cells carrying mutations for cdc27 and bub3 suggest the existence of differentially activated APC/C complexes. Altogether, our data support the hypothesis that the mitotic checkpoint protein Bub3 is also required to regulate entry and progression through early stages of mitosis.

Key words: Drosophila, Mitosis, Checkpoint, Bub3, APC, Cyclosome

This article has been cited by other articles:

				E. Logarinho, T. Resende, C. Torres, and H. Bousbaa The Human Spindle Assembly Checkpoint Protein Bub3 Is Required for the Establishment of Efficient Kinetochore-Microtubule Attachments Mol. Biol. Cell, April 1, 2008; 19(4): 1798 - 1813. [Abstract] [Full Text] [PDF]

				H. Muller, M.-L. Fogeron, V. Lehmann, H. Lehrach, and B. M. H. Lange Response to Comments on "A Centrosome-Independent Role for {gamma}-TuRC Proteins in the Spindle Assembly Checkpoint" Science, May 18, 2007; 316(5827): 982d - 982d. [Abstract] [Full Text] [PDF]

				K. W.-H. Lo, J. M. Kogoy, and K. K. Pfister The DYNLT3 Light Chain Directly Links Cytoplasmic Dynein to a Spindle Checkpoint Protein, Bub3 J. Biol. Chem., April 13, 2007; 282(15): 11205 - 11212. [Abstract] [Full Text] [PDF]

				B. Orr, H. Bousbaa, and C. E. Sunkel Mad2-independent Spindle Assembly Checkpoint Activation and Controlled Metaphase-Anaphase Transition in Drosophila S2 Cells Mol. Biol. Cell, March 1, 2007; 18(3): 850 - 863. [Abstract] [Full Text] [PDF]

				J. R. Wood, D. A. Dumesic, D. H. Abbott, and J. F. Strauss III Molecular Abnormalities in Oocytes from Women with Polycystic Ovary Syndrome Revealed by Microarray Analysis J. Clin. Endocrinol. Metab., February 1, 2007; 92(2): 705 - 713. [Abstract] [Full Text] [PDF]

				A. Sakaguchi and R. Steward Aberrant monomethylation of histone H4 lysine 20 activates the DNA damage checkpoint in Drosophila melanogaster J. Cell Biol., January 16, 2007; 176(2): 155 - 162. [Abstract] [Full Text] [PDF]