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First published online 24 May 2005
doi: 10.1242/jcs.02393

Journal of Cell Science 118, 2637-2648 (2005)
Published by The Company of Biologists 2005
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Research Article

The formin family protein CaBni1p has a role in cell polarity control during both yeast and hyphal growth in Candida albicans

Chang Run Li, Yan Ming Wang, Xin De Zheng, Hui Yan Liang, Jason Chih Wei Tang and Yue Wang*

Molecular and Cell Biology Laboratory, Institute of Molecular and Cell Biology, 61 Bioplis Drive, Singapore 138673, Republic of Singapore

* Author for correspondence (e-mail: mcbwangy{at}imcb.a-star.edu.sg)

Accepted 21 March 2005

Formins are conserved eukaryotic proteins playing key roles in regulating cell polarity. We have characterized the roles of a formin CaBni1p in the polymorphic fungus Candida albicans. CaBni1p localized persistently to hyphal tips during hyphal growth but to distinct growth sites at different cell cycle stages during yeast growth. Cabni1{Delta} yeast cells exhibited several morphological defects, such as round and enlarged cells, widened bud necks and a random budding pattern. Although Cabni1{Delta} cells could still undergo yeast-hypha growth switch, the hyphae were markedly swollen. Cabni1{Delta} also showed defects in spindle and cytoplasmic microtubule orientation and positioning. Coincidentally, the spindle orientation protein CaKar9p in Cabni1{Delta} yeast cells appeared as multiple random cortical spots, in contrast to the single spot at the bud tip of many wild-type cells. Interestingly, several defects manifested in Cabni1{Delta} yeast cells were partially corrected during hyphal growth. We found that the second formin CaBnr1p was recruited to hyphal tips, while it localized only to the bud neck during yeast growth. This behavior of CaBnr1p may play a key role in correcting Cabni1{Delta} defects during hyphal growth. Cabni1{Delta} exhibited reduced virulence in mice. These results indicate that the formins play an important role in Candida albicans polarized growth and CaBni1p's function is required for virulence.

Key words: Polarized Growth, Actin, Cytoplasmic microtubule, Kar9p, Fungal infection


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