spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online June 23, 2005
doi: 10.1242/10.1242/jcs.02439

Journal of Cell Science 118, 2849-2858 (2005)
Published by The Company of Biologists 2005
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marzesco, A.-M.
Right arrow Articles by Huttner, W. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marzesco, A.-M.
Right arrow Articles by Huttner, W. B.

Research Article

Release of extracellular membrane particles carrying the stem cell marker prominin-1 (CD133) from neural progenitors and other epithelial cells

Anne-Marie Marzesco1, Peggy Janich1, Michaela Wilsch-Bräuninger1, Véronique Dubreuil1, Katja Langenfeld1, Denis Corbeil1,2,* and Wieland B. Huttner1,*

1 Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
2 Medical Clinic and Polyclinic I, Dresden University of Technology, Fetscherstrasse 74, 01307 Dresden, Germany

* Authors for correspondence (e-mail: corbeil{at}biotec.tu-dresden.de and huttner{at}mpi-cbg.de)

Accepted 14 April 2005

Apical plasma membrane constituents of mammalian neural stem/progenitor cells have recently been implicated in maintaining their stem/progenitor cell state. Here, we report that in the developing embryonic mouse brain, the fluid in the lumen of the neural tube contains membrane particles carrying the stem cell marker prominin-1 (CD133), a pentaspan membrane protein found on membrane protrusions of the apical surface of neuroepithelial cells. Two size classes of prominin-1-containing membrane particles were observed in the ventricular fluid: {approx}600-nm particles, referred to as P2 particles, and 50-80-nm vesicles, referred to as P4 particles. The P2 and P4 particles appeared in the ventricular fluid at the very onset and during the early phase of neurogenesis, respectively. Concomitant with their appearance, the nature of the prominin-1-containing apical plasma membrane protrusions of neuroepithelial cells changed, in that microvilli were lost and large pleiomorphic protuberances appeared. P4 particles were found in various body fluids of adult humans, including saliva, seminal fluid and urine, and were released by the epithelial model cell line Caco-2 upon differentiation. Importantly, P4 particles were distinct from exosomes. Our results demonstrate the widespread occurrence of a novel class of extracellular membrane particles containing proteins characteristic of stem cells, and raise the possibility that the release of the corresponding membrane subdomains from the apical surface of neural progenitors and other epithelial cells may have a role in tissue development and maintenance. Moreover, the presence of prominin-1-containing membrane particles in human body fluids may provide the basis for a protein-based diagnosis of certain diseases.

Key words: extracellular membranes particles, neuroepithelium, stem cells, epithelia, prominin-1, CD133




This article has been cited by other articles:


Home page
 Stem CellsHome page
H. B. Huttner, P. Janich, M. Kohrmann, J. Jaszai, F. Siebzehnrubl, I. Blumcke, M. Suttorp, M. Gahr, D. Kuhnt, C. Nimsky, et al.
The Stem Cell Marker Prominin-1/CD133 on Membrane Particles in Human Cerebrospinal Fluid Offers Novel Approaches for Studying Central Nervous System Disease
Stem Cells, March 1, 2008; 26(3): 698 - 705.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. Coskun, H. Wu, B. Blanchi, S. Tsao, K. Kim, J. Zhao, J. C. Biancotti, L. Hutnick, R. C. Krueger Jr., G. Fan, et al.
From the Cover: CD133+ neural stem cells in the ependyma of mammalian postnatal forebrain
PNAS, January 22, 2008; 105(3): 1026 - 1031.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
J. Pruszak, K.-C. Sonntag, M. H. Aung, R. Sanchez-Pernaute, and O. Isacson
Markers and Methods for Cell Sorting of Human Embryonic Stem Cell-Derived Neural Cell Populations
Stem Cells, September 1, 2007; 25(9): 2257 - 2268.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. Lagares, H.-Y. Li, X.-F. Zhou, and C. Avendano
Primary Sensory Neuron Addition in the Adult Rat Trigeminal Ganglion: Evidence for Neural Crest Glio-Neuronal Precursor Maturation
J. Neurosci., July 25, 2007; 27(30): 7939 - 7953.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
M. Yanagisawa and R. K Yu
The expression and functions of glycoconjugates in neural stem cells
Glycobiology, July 1, 2007; 17(7): 57R - 74R.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
V. Dubreuil, A.-M. Marzesco, D. Corbeil, W. B. Huttner, and M. Wilsch-Brauninger
Midbody and primary cilium of neural progenitors release extracellular membrane particles enriched in the stem cell marker prominin-1
J. Cell Biol., February 12, 2007; 176(4): 483 - 495.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
E.A. Campbell, L. O'Hara, R.D. Catalano, A.M. Sharkey, T.C. Freeman, and M. H. Johnson
Temporal expression profiling of the uterine luminal epithelium of the pseudo-pregnant mouse suggests receptivity to the fertilized egg is associated with complex transcriptional changes
Hum. Reprod., October 1, 2006; 21(10): 2495 - 2513.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005