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First published online June 23, 2005
doi: 10.1242/10.1242/jcs.02424

Journal of Cell Science 118, 2891-2899 (2005)
Published by The Company of Biologists 2005
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Research Article

PHR1, an integral membrane protein of the inner ear sensory cells, directly interacts with myosin 1c and myosin VIIa

Raphaël Etournay1,*, Aziz El-Amraoui1,*, Amel Bahloul1, Stéphane Blanchard1, Isabelle Roux1, Guillaume Pézeron1, Nicolas Michalski1, Laurent Daviet2, Jean-Pierre Hardelin1, Pierre Legrain3 and Christine Petit1,{ddagger}

1 Unité de Génétique des Déficits Sensoriels, INSERM U587, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France
2 Hybrigenics, 3-5 impasse Reille, 75014 Paris, France
3 Département de Biologie Joliot-Curie, CEA, 91191 Gif-sur-Yvette, France

{ddagger} Author for correspondence (e-mail: cpetit{at}pasteur.fr)

Accepted 6 April 2005

By using the yeast two-hybrid technique, we identified a candidate protein ligand of the myosin 1c tail, PHR1, and found that this protein can also bind to the myosin VIIa tail. PHR1 is an integral membrane protein that contains a pleckstrin homology (PH) domain. Myosin 1c and myosin VIIa are two unconventional myosins present in the inner ear sensory cells. We showed that PHR1 immunoprecipitates with either myosin tail by using protein extracts from cotransfected HEK293 cells. In vitro binding assays confirmed that PHR1 directly interacts with these two myosins. In both cases the binding involves the PH domain. In vitro interactions between PHR1 and the myosin tails were not affected by the presence or absence of Ca2+ and calmodulin. Finally, we found that PHR1 is able to dimerise. As PHR1 is expressed in the vestibular and cochlear sensory cells, its direct interactions with the myosin 1c and VIIa tails are likely to play a role in anchoring the actin cytoskeleton to the plasma membrane of these cells. Moreover, as both myosins have been implicated in the mechanotransduction slow adaptation process that takes place in the hair bundles, we propose that PHR1 is also involved in this process.

Key words: Myosin 1c, Myosin VIIa, PHR1, Inner ear, Hair cell


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