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First published online 19 July 2005
doi: 10.1242/jcs.02453

Journal of Cell Science 118, 3289-3304 (2005)
Published by The Company of Biologists 2005
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Research Article

Characteristics of subepithelial fibroblasts as a mechano-sensor in the intestine: cell-shape-dependent ATP release and P2Y1 signaling

Kishio Furuya1,*, Masahiro Sokabe1,2 and Sonoko Furuya3,4

1 Cell Mechano-Sensing Project, ICORP and SORST, Japan Science and Technology Agency, Nagoya, 466-8550, Japan
2 Department of Physiology, Nagoya University School of Medicine, Nagoya, 466-8550, Japan
3 Section of Brain Structure, Center for Brain Experiment, Okazaki 444-8585, Japan
4 The Graduate University for Advanced Studies, National Institute for Physiological Sciences, Okazaki 444-8585, Japan

* Author for correspondence (e-mail: furuya{at}med.nagoya-u.ac.jp)

Accepted 22 April 2005

Subepithelial fibroblasts form a cellular network just under the epithelium of the gastrointestinal tract. Using primary cultured cells isolated from rat duodenal villi, we previously found that subepithelial fibroblasts reversibly changed cell morphology between flat and stellate-shape depending on intracellular cAMP levels. In this paper, we examined cell-cell communication via released ATP and Ca2+ signaling in the cellular network. Subepithelial fibroblasts were sensitive to mechanical stress such as `touching' a cell with a fine glass rod and `stretching' cells cultured on elastic silicone chamber. Mechanical stimulations evoked Ca2+-increase in the cells and ATP-release from the cells. The released ATP activated P2Y receptors on the surrounding cells and propagated Ca2+-waves through the network. Concomitant with Ca2+-waves, a transient contraction of the network was observed. Histochemical, RT-PCR, western blotting and Ca2+ response analyses indicated P2Y1 is a dominant functional subtype. ATP-release and Ca2+ signaling were cell-shape dependent, i.e. they were abolished in stellate-shaped cells treated with dBcAMP, and recovered or further enhanced in re-flattened cells treated with endothelin. The response to ATP also decreased in stellate-shaped cells. These findings indicate cAMP-mediated intracellular signaling causes cell-shape change, which accompanies the changes in mechano- and ATP sensitivities. Using a co-culture system of neuronal cells (NG108-15) with subepithelial fibroblasts, we confirmed that mechanically induced Ca2+-waves propagated to neurons. From these findings we propose that subepithelial fibroblasts work as a mechanosensor in the intestine. Uptake of food, water and nutrients may cause mechanical stress on subepithelial fibroblasts in the villi. The ATP released by mechanical stimulation elicits Ca2+-wave propagation through the network via P2Y1 activation and also activates P2X on terminals of mucosal sensory neurons to regulate peristaltic motility.

Key words: Subepithelial fibroblasts, Mechanosensing, Intestine, ATP release, P2Y, Myofibroblasts


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