|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online 26 July 2005
doi: 10.1242/jcs.02502
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Article |
1 Center for Basic Neuroscience, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390-9111, USA
2 University of Zürich, Institute of Physiology, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland
* Author for correspondence (e-mail: hkrame{at}mednet.swmed.edu)
Accepted 17 May 2005
Mutations that disrupt trafficking to lysosomes and lysosome-related organelles cause multiple diseases, including Hermansky-Pudlak syndrome. The Drosophila eye is a model system for analyzing such mutations. The eye-color genes carnation and deep orange encode two subunits of the Vps-C protein complex required for endosomal trafficking and pigment-granule biogenesis. Here we demonstrate that dVps16A (CG8454) encodes another Vps-C subunit. Biochemical experiments revealed a specific interaction between the dVps16A C-terminus and the Sec1/Munc18 homolog Carnation but not its closest homolog, dVps33B. Instead, dVps33B interacted with a related protein, dVps16B (CG18112). Deep orange bound both Vps16 homologs. Like a deep orange null mutation, eye-specific RNAi-induced knockdown of dVps16A inhibited lysosomal delivery of internalized ligands and interfered with biogenesis of pigment granules. Ubiquitous knockdown of dVps16A was lethal. Together, these findings demonstrate that Drosophila Vps16A is essential for lysosomal trafficking. Furthermore, metazoans have two types of Vps-C complexes with non-redundant functions.
Key words: Endocytic trafficking, Vps-C genes, Pigment granules, Lysosome-related organelles, Autophagosomes
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  C. G. Angers and A. J. Merz HOPS Interacts with Apl5 at the Vacuole Membrane and Is Required for Consumption of AP-3 Transport Vesicles Mol. Biol. Cell, November 1, 2009; 20(21): 4563 - 4574. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Simonsen and S. A. Tooze Coordination of membrane events during autophagy by multiple class III PI3-kinase complexes J. Cell Biol., September 21, 2009; 186(6): 773 - 782. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kaido, H. Wada, M. Shindo, and S. Hayashi Essential requirement for RING finger E3 ubiquitin ligase Hakai in early embryonic development of Drosophila Genes Cells, September 1, 2009; 14(9): 1067 - 1077. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. E. Rusten and H. Stenmark How do ESCRT proteins control autophagy? J. Cell Sci., July 1, 2009; 122(13): 2179 - 2183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Akbar, S. Ray, and H. Kramer The SM Protein Car/Vps33A Regulates SNARE-mediated Trafficking to Lysosomes and Lysosome-related Organelles Mol. Biol. Cell, March 15, 2009; 20(6): 1705 - 1714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-d. Zhu, G. Salazar, S. A. Zlatic, B. Fiza, M. M. Doucette, C. J. Heilman, A. I. Levey, V. Faundez, and S. W. L'Hernault SPE-39 Family Proteins Interact with the HOPS Complex and Function in Lysosomal Delivery Mol. Biol. Cell, February 1, 2009; 20(4): 1223 - 1240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Lim and R. Kraut The Drosophila BEACH Family Protein, Blue Cheese, Links Lysosomal Axon Transport with Motor Neuron Degeneration J. Neurosci., January 28, 2009; 29(4): 951 - 963. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Toda, H. Mochizuki, R. Flores III, R. Josowitz, T. B. Krasieva, V. J. LaMorte, E. Suzuki, J. G. Gindhart, K. Furukubo-Tokunaga, and T. Tomoda UNC-51/ATG1 kinase regulates axonal transport by mediating motor-cargo assembly Genes & Dev., December 1, 2008; 22(23): 3292 - 3307. [Abstract] [Full Text] [PDF]
|
|
