QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 6 September 2005
doi: 10.1242/jcs.02555

This Article Figures Only Full Text Full Text (PDF) All Versions of this Article: jcs.02555v1 118/19/4343    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rosu-Myles, M. Articles by Bhatia, M. Search for Related Content PubMed PubMed Citation Articles by Rosu-Myles, M. Articles by Bhatia, M. Social Bookmarking                         What's this?

A unique population of bone marrow cells migrates to skeletal muscle via hepatocyte growth factor/c-met axis

¹ Robarts Research Institute, Krembil Centre for Stem Cell Biology and Regenerative Medicine, 100 Perth Drive, London, Ontario, N6A 5K8, Canada
² The University of Western Ontario, Department of Microbiology and Immunology, Toronto, Ontario, Canada
³ University of Toronto, Department of Bioengineering, Toronto, Ontario, Canada

^* Author for correspondence (e-mail: mbhatia{at}robarts.ca)

Accepted 21 June 2005

Cells expressing the CD45-associated hematopoietic marker are predominantly present in the mammalian bone marrow (BM), but have recently been shown to also reside in the skeletal muscle and potentially participate in muscle repair. Despite the consistent observations, the specific relationship and potential migration of CD45⁺ cells in the BM versus CD45⁺ cells residing in the muscle remain unclear, in addition to any understanding of the factors that may regulate the trafficking of CD45⁺-derived BM cells to skeletal muscle upon i.v. transplantation. Here, transplantation of BM-derived cells fully replaced the CD45⁺ fraction of skeletal muscle, but gave rise to progenitor cells with distinct hematopoietic lineage capacity from CD45⁺ cells residing in the BM. Using transwell migration assays, a subset of BM cells was shown to migrate exclusively to mature skeletal muscle cells and not BM-derived stromal cells. Unlike migration of BM cells to stroma, myofiber induced migration of BM-derived cells was not affected by stromal-derived factor-1 (SDF-1) neutralization or CXCR4-blocking antibody, but could be reduced by addition of c-met-blocking antibody and augmented by hepatocyte growth factor (HGF), the putative ligand for c-met. We suggest that the BM compartment consists of a functionally complex population of CD45⁺ progenitors that includes a subset of HGF/c-met responsive cells capable of migration to skeletal muscle. This previously unappreciated basis for cellular tracking now aids in defining regulatory networks that distinguish the stem cell niche of the BM versus skeletal muscle microenvironments.

Key words: Hematopoietic stem cells, Bone marrow, Skeletal muscle, Migration

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Related articles in JCS:

CD45 flexes its muscles

JCS 2005 118: 1903. [Full Text]  

This article has been cited by other articles:

				T. Trapp, G. Kogler, A. El-Khattouti, R. V. Sorg, M. Besselmann, M. Focking, C. P. Buhrle, I. Trompeter, J. C. Fischer, and P. Wernet Hepatocyte Growth Factor/c-MET Axis-mediated Tropism of Cord Blood-derived Unrestricted Somatic Stem Cells for Neuronal Injury J. Biol. Chem., November 21, 2008; 283(47): 32244 - 32253. [Abstract] [Full Text] [PDF]

				S. Dimmeler and A. Leri Aging and Disease as Modifiers of Efficacy of Cell Therapy Circ. Res., June 6, 2008; 102(11): 1319 - 1330. [Abstract] [Full Text] [PDF]

				B. G. Galvez, M. Sampaolesi, S. Brunelli, D. Covarello, M. Gavina, B. Rossi, G. Costantin, Y. Torrente, and G. Cossu Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability J. Cell Biol., July 17, 2006; 174(2): 231 - 243. [Abstract] [Full Text] [PDF]