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First published online January 14, 2005
doi: 10.1242/10.1242/jcs.01630

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Remodelling of the nuclear lamina and nucleoskeleton is required for skeletal muscle differentiation in vitro

Ewa Markiewicz, Maria Ledran^* and Christopher J. Hutchison

Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, University of Durham, South Road, Durham, DH1 4EB, UK

Author for correspondence (e-mail: c.j.hutchison{at}durham.ac.uk)

Accepted 3 November 2004

Changes in the expression and distribution of nuclear lamins were investigated during C2C12 myoblast differentiation. The expression of most lamins was unchanged during myogenesis. By contrast, lamin-B₂ expression increased and LAP2 expression decreased twofold. These changes were correlated with reduced solubility and redistribution of A-type lamins. When C2C12 myoblasts were transfected with a lamin-A mutant that causes autosomal dominant Emery-Dreifuss muscular dystrophy (AD-EDMD), the mutant protein accumulated in the nucleoplasm and exerted dominant influences over endogenous lamins. Myoblasts transfected with wild-type lamins differentiated, albeit more slowly, whereas myoblasts transfected with mutant lamins failed to differentiate. Myoblast differentiation requires dephosphorylation of the retinoblastoma protein Rb. During myogenesis, Rb was rapidly and progressively dephosphorylated. Underphosphorylated Rb formed complexes with LAP2 in proliferating myoblasts and postmitotic myoblasts. In myoblasts transfected with the mutant lamins, this complex was disrupted. These data suggest that remodelling of the nucleoskeleton is necessary for skeletal-muscle differentiation and for correct regulation of Rb pathways.

Key words: Lamins, Laminopathy, Myogenesis, LAP2, Nucleoskeleton

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