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First published online October 27, 2005
doi: 10.1242/10.1242/jcs.02622

Journal of Cell Science 118, 5101-5108 (2005)
Published by The Company of Biologists 2005
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Research Article

Phosphorylation of Nlp by Plk1 negatively regulates its dynein-dynactin-dependent targeting to the centrosome

Martina Casenghi, Francis A. Barr*,{ddagger} and Erich A. Nigg*,{ddagger}

Max-Planck Institute of Biochemistry, Department of Cell Biology, Am Klopferspitz 18, 82152 Martinsried, Germany

{ddagger} Authors for correspondence (e-mail: barr{at}biochem.mpg.de; nigg{at}biochem.mpg.de)

Accepted 3 August 2005

When cells enter mitosis the microtubule (MT) network undergoes a profound rearrangement, in part due to alterations in the MT nucleating and anchoring properties of the centrosome. Ninein and the ninein-like protein (Nlp) are centrosomal proteins involved in MT organisation in interphase cells. We show that the overexpression of these two proteins induces the fragmentation of the Golgi, and causes lysosomes to disperse toward the cell periphery. The ability of Nlp and ninein to perturb the cytoplasmic distribution of these organelles depends on their ability to interact with the dynein-dynactin motor complex. Our data also indicate that dynactin is required for the targeting of Nlp and ninein to the centrosome. Furthermore, phosphorylation of Nlp by the polo-like kinase 1 (Plk1) negatively regulates its association with dynactin. These findings uncover a mechanism through which Plk1 helps to coordinate changes in MT organisation with cell cycle progression, by controlling the dynein-dynactin-dependent transport of centrosomal proteins.

Key words: Dynein, Dynactin, Plk1, Nlp, Centrosome




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© The Company of Biologists Ltd 2005