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First published online November 23, 2005
doi: 10.1242/10.1242/jcs.02658

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SV2A and SV2C are not vesicular Ca²⁺ transporters but control glucose-evoked granule recruitment

¹ Department of Cell Physiology and Metabolism, University Medical Center, 1 rue Michel-Servet, 1211 Geneva 4, Switzerland
² W.M. Keck Center for Learning and Memory, Department of Neurobiology and Anatomy, University of Texas-Houston Medical School, 6431 Fannin Street, Houston, TX 77030, USA

^* Author for correspondence (e-mail: claes.wollheim{at}medecine.unige.ch)

Accepted 24 August 2005

Synaptic vesicle protein 2 (SV2) is expressed in neuroendocrine cells as three homologous isoforms, SV2A, SV2B and SV2C. Ca²⁺-dependent function in exocytosis has been attributed to SV2A and SV2B, without elucidation of the mechanism. The role of SV2C has not yet been addressed. Here we characterize the three SV2 isoforms and define their involvement in regulated insulin secretion. SV2A and SV2C are associated with insulin-containing granules and synaptic-like-microvesicles (SLM) in INS-1E insulinoma and primary ß-cells, whereas SV2B is only present on SLM. Neither overexpression nor isoform-specific silencing of SV2A or SV2C by RNA interference modifies depolarization-triggered cytosolic [Ca²⁺] rises or secretory granule [Ca²⁺], measured with a VAMP-2 aequorin chimera. This strongly argues against any Ca²⁺ transport function of SV2. Moreover, up- or downregulation of these isoforms has no influence on K⁺-induced insulin release suggesting that SV2 does not affect the Ca²⁺-dependent step(s) of exocytosis. By contrast, glucose-elicited secretion is inhibited during the sustained rather than the early phase, placing the action of SV2 on the recruitment of granules from the reserve pool to the plasma membrane. This conclusion is reinforced by capacitance measurements in glucose-stimulated SV2C-deficient cells. Like capacitance, evoked and basal hormone release are attenuated more by silencing of SV2C compared with SV2A. This indicates only partial redundancy and highlights a key role for SV2C in the secretory process.

Key words: INS-1E cells, Insulin release, Granular and cytosolic calcium, Membrane capacitance, RNA interference

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