spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 25 January 2005
doi: 10.1242/jcs.01647

Journal of Cell Science 118, 781-793 (2005)
Published by The Company of Biologists 2005
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrowOA All Versions of this Article:
jcs.01647v1
118/4/781    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JCS
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jang, S.-I.
Right arrow Articles by Steinert, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jang, S.-I.
Right arrow Articles by Steinert, P. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Characterization of human epiplakin: RNAi-mediated epiplakin depletion leads to the disruption of keratin and vimentin IF networks

Shyh-Ing Jang*,{ddagger},§, Alexandr Kalinin*, Kaoruko Takahashi, Lyuben N. Marekov and Peter M. Steinert

Laboratory of Skin Biology, NIAMS, National Institutes of Health, Bethesda, MD 20892-8023, USA

§ Author for correspondence (e-mail: jangs{at}mail.nih.gov)

Accepted 16 November 2004

Epiplakin is a member of the plakin family with multiple copies of the plakin repeat domain (PRD). We studied the subcellular distribution and interactions of human epiplakin by immunostaining, overlay assays and RNAi knockdown. Epiplakin decorated the keratin intermediate filaments (IF) network and partially that of vimentin. In the binding assays, the repeat unit (PRD plus linker) showed strong binding and preferentially associated with assembled IF over keratin monomers. Epiplakin knockdown revealed disruption of IF networks in simple epithelial but not in epidermal cells. In rescue experiments, the repeat unit was necessary to prevent the collapse of IF networks in transient knockdown; however, it could only partially restore the keratin but not the vimentin IF network in stably knocked down HeLa cells. We suggest that epiplakin is a cytolinker involved in maintaining the integrity of IF networks in simple epithelial cells. Furthermore, we observed an increase of epiplakin expression in keratinocytes after the calcium switch, suggesting the involvement of epiplakin in the process of keratinocyte differentiation.

Key words: epiplakin, intermediate filaments, keratinocyte


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related articles in JCS:

Epiplakin needed for network maintenance

JCS 2005 118: 403. [Full Text]  



This article has been cited by other articles:


Home page
 GENES CELLSHome page
T. Yoshida, N. Shiraki, H. Baba, M. Goto, S. Fujiwara, K. Kume, and S. Kume
Expression patterns of epiplakin1 in pancreas, pancreatic cancer and regenerating pancreas.
Genes Cells, July 1, 2008; 13(7): 667 - 678.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. Spazierer, J. Raberger, K. Gross, P. Fuchs, and G. Wiche
Stress-induced recruitment of epiplakin to keratin networks increases their resistance to hyperphosphorylation-induced disruption
J. Cell Sci., March 15, 2008; 121(6): 825 - 833.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.-I. Jang, E.-J. Lee, P. S. Hart, M. Ramaswami, D. Pallos, and T. C. Hart
Germ Line Gain of Function with SOS1 Mutation in Hereditary Gingival Fibromatosis
J. Biol. Chem., July 13, 2007; 282(28): 20245 - 20255.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. A. Long, V. Boczonadi, L. McInroy, M. Goldberg, and A. Maatta
Periplakin-dependent re-organisation of keratin cytoskeleton and loss of collective migration in keratin-8-downregulated epithelial sheets
J. Cell Sci., December 15, 2006; 119(24): 5147 - 5159.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Goto, H. Sumiyoshi, T. Sakai, R. Fassler, S. Ohashi, E. Adachi, H. Yoshioka, and S. Fujiwara
Elimination of Epiplakin by Gene Targeting Results in Acceleration of Keratinocyte Migration in Mice
Mol. Cell. Biol., January 15, 2006; 26(2): 548 - 558.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
D. Spazierer, P. Fuchs, S. Reipert, I. Fischer, M. Schmuth, H. Lassmann, and G. Wiche
Epiplakin Is Dispensable for Skin Barrier Function and for Integrity of Keratin Network Cytoarchitecture in Simple and Stratified Epithelia
Mol. Cell. Biol., January 15, 2006; 26(2): 559 - 568.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005