spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online February 23, 2005
doi: 10.1242/10.1242/jcs.01694

Journal of Cell Science 118, 1071-1080 (2005)
Published by The Company of Biologists 2005
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pujol, F.
Right arrow Articles by Boudin, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pujol, F.
Right arrow Articles by Boudin, H.

Research Article

The chemokine SDF-1 differentially regulates axonal elongation and branching in hippocampal neurons

Fabien Pujol, Patrick Kitabgi and Hélène Boudin*,{ddagger}

INSERM E0350, Hospital St Antoine, 184 rue du Fg St Antoine, 75571 Paris CEDEX 12, France

{ddagger} Author for correspondence (e-mail: helene.boudin{at}univ-nantes.fr)

Accepted 30 December 2004

Recent data have shown that the chemokine SDF-1 plays a critical role in several aspects of brain development such as cell migration and axon pathfinding. However, its potential function in the generation of axons and dendrites is poorly characterized. In order to better understand the role of SDF-1 in the development of central neurons, we studied the cellular distribution of the SDF-1 receptor CXCR4 by immunocytochemistry of developing hippocampal neurons and tested the effect of SDF-1 in process patterning at the early stages of neuronal development. We found that CXCR4 immunoreactivity undergoes a striking redistribution during development. At the early stages, from day 2 to day 4 in culture, CXCR4 is particularly concentrated at the leading edge of growing neurites. As the cells mature, staining declines at the tip of the processes and becomes more broadly distributed along axons and, to a lesser extent, dendrites. SDF-1 stimulation of neurons at day 1-2 in culture triggers several effects on neuronal morphogenesis. SDF-1 reduces growth cone number and axonal outgrowth but stimulates axonal branching. These latter two effects are not observed in other neurites. This study unravels a new role for SDF-1/CXCR4 in specifying hippocampal neuron morphology by regulating axonal patterning at an early stage of neuronal development.

Key words: Hippocampal neuron, Axon, Development, Chemokine, Cell culture




This article has been cited by other articles:


Home page
 Neurorehabil Neural RepairHome page
H. Shichinohe, S. Kuroda, S. Tsuji, S. Yamaguchi, S. Yano, J.-B. Lee, H. Kobayashi, S. Kikuchi, K. Hida, and Y. Iwasaki
Bone Marrow Stromal Cells Promote Neurite Extension in Organotypic Spinal Cord Slice: Significance for Cell Transplantation Therapy
Neurorehabil Neural Repair, September 1, 2008; 22(5): 447 - 457.
[Abstract] [PDF]

Home page
 J. Neurosci.Home page
Y. Ohshima, T. Kubo, R. Koyama, M. Ueno, M. Nakagawa, and T. Yamashita
Regulation of Axonal Elongation and Pathfinding from the Entorhinal Cortex to the Dentate Gyrus in the Hippocampus by the Chemokine Stromal Cell-Derived Factor 1{alpha}
J. Neurosci., August 13, 2008; 28(33): 8344 - 8353.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
B. J. Bhattacharyya, G. Banisadr, H. Jung, D. Ren, D. G. Cronshaw, Y. Zou, and R. J. Miller
The Chemokine Stromal Cell-Derived Factor-1 Regulates GABAergic Inputs to Neural Progenitors in the Postnatal Dentate Gyrus
J. Neurosci., June 25, 2008; 28(26): 6720 - 6730.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. J. Baudouin, J. Angibaud, G. Loussouarn, V. Bonnamain, A. Matsuura, M. Kinebuchi, P. Naveilhan, and H. Boudin
The Signaling Adaptor Protein CD3{zeta} Is a Negative Regulator of Dendrite Development in Young Neurons
Mol. Biol. Cell, June 1, 2008; 19(6): 2444 - 2456.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. Callewaere, B. Fernette, D. Raison, P. Mechighel, A. Burlet, A. Calas, P. Kitabgi, S. M. Parsadaniantz, and W. Rostene
Cellular and Subcellular Evidence for Neuronal Interaction between the Chemokine Stromal Cell-Derived Factor-1/CXCL 12 and Vasopressin: Regulation in the Hypothalamo-Neurohypophysial System of the Brattleboro Rats
Endocrinology, January 1, 2008; 149(1): 310 - 319.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
R. Stumm and V. Hollt
CXC chemokine receptor 4 regulates neuronal migration and axonal pathfinding in the developing nervous system: implications for neuronal regeneration in the adult brain
J. Mol. Endocrinol., March 1, 2007; 38(3): 377 - 382.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. E. McCandless, Q. Wang, B. M. Woerner, J. M. Harper, and R. S. Klein
CXCL12 Limits Inflammation by Localizing Mononuclear Infiltrates to the Perivascular Space during Experimental Autoimmune Encephalomyelitis
J. Immunol., December 1, 2006; 177(11): 8053 - 8064.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005