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First published online 22 March 2005
doi: 10.1242/jcs.02276

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Classical protein kinase C(s) regulates targeting of synaptotagmin IX to the endocytic recycling compartment

Yael Haberman^1,*, Idit Ziv¹, Yaara Gorzalczany¹, Mitsunori Fukuda² and Ronit Sagi-Eisenberg^1,

¹ Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
² Fukuda Initiative Research Unit, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan

Author for correspondence (e-mail: histol3{at}post.tau.ac.il)

Accepted 20 January 2005

Neuronal and non-neuronal tissues show distinctly different intracellular localization of synaptotagmin (Syt) homologues. Therefore, cell type-specific mechanisms are likely to direct Syt homologues to their final cellular destinations. Syt IX localizes to dense core vesicles in PC12 cells. However, in the rat basophilic leukemia (RBL-2H3) mast cell line, as well as in CHO cells, Syt IX is localized at the endocytic recycling compartment (ERC). We show that targeting of Syt IX to the ERC involves constitutive trafficking to the plasma membrane followed by internalization and transport to the ERC. We further show that internalization from the plasma membrane and delivery to the ERC are dependent on phosphorylation by Ca²⁺-dependent protein kinase C or ß. As such, correct targeting of Syt IX is facilitated by the phorbol ester TPA but prevented by the cPKC inhibitor Go 6976.

Key words: Endocytic recycling compartment (ERC), Endocytosis, Synaptotagmin, Protein kinase C, RBL-2H3 mast cells

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