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First published online 29 March 2005
doi: 10.1242/jcs.02305

Journal of Cell Science 118, 1705-1714 (2005)
Published by The Company of Biologists 2005
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Research Article

The nuclear rim protein Amo1 is required for proper microtubule cytoskeleton organisation in fission yeast

Mercedes Pardo1,*,{ddagger} and Paul Nurse2

1 Cell Cycle Laboratory, Cancer Research UK, 44 Lincoln's Inn Fields, London, WC2A 3PX, UK
2 The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA

{ddagger} Corresponding author (e-mail: mp3{at}sanger.ac.uk)

Accepted 3 February 2005

Microtubules have a central role in cell division and cell polarity in eukaryotic cells. The fission yeast is a useful organism for studying microtubule regulation owing to the highly organised nature of its microtubular arrays. To better understand microtubule dynamics and organisation we carried out a screen that identified over 30 genes whose overexpression resulted in microtubule cytoskeleton abnormalities. Here we describe a novel nucleoporin-like protein, Amo1, identified in this screen. Amo1 localises to the nuclear rim in a punctate pattern that does not overlap with nuclear pore complex components. Amo1{Delta} cells are bent, and they have fewer microtubule bundles that curl around the cell ends. The microtubules in amo1{Delta} cells have longer dwelling times at the cell tips, and grow in an uncoordinated fashion. Lack of Amo1 also causes a polarity defect. Amo1 is not required for the microtubule loading of several factors affecting microtubule dynamics, and does not seem to be required for nuclear pore function.

Key words: Microtubules, Fission yeast, Nuclear pore complex


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