QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 13 December 2005
doi: 10.1242/jcs.02737

This Article Figures Only Full Text Full Text (PDF) Supplementary Material All Versions of this Article: jcs.02737v1 119/1/104    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nishimura, Y. Articles by Yonemura, S. Search for Related Content PubMed PubMed Citation Articles by Nishimura, Y. Articles by Yonemura, S.

Centralspindlin regulates ECT2 and RhoA accumulation at the equatorial cortex during cytokinesis

Laboratory for Cellular Morphogenesis, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan

^* Author for correspondence (e-mail: yonemura{at}cdb.riken.jp)

Accepted 4 October 2005

During determination of the cell division plane, an actomyosin contractile ring is induced at the equatorial cell cortex by signals from the mitotic apparatus and contracts to cause cleavage furrow progression. Although the small GTPase RhoA is known to regulate the progression, probably by controlling actin filament assembly and enhancing actomyosin interaction, any involvement of RhoA in division plane determination is unknown. In this study, using a trichloroacetic acid (TCA) fixation protocol we recently developed, we show that RhoA accumulates at the equatorial cortex before furrow initiation and continues to concentrate at the cleavage furrow during cytokinesis. We also demonstrate that both Rho activity and microtubule organization are required for RhoA localization and proper furrowing. Selective disruption of microtubule organization revealed that both astral and central spindle microtubules can recruit RhoA at the equatorial cortex. We find that centralspindlin and ECT2 are required for RhoA localization and furrowing. Centralspindlin is localized both to central spindle microtubules and at the tips of astral microtubules near the equatorial cortex and recruits ECT2. Positional information for division plane determination from microtubules is transmitted to the cell cortex to organize actin cytoskeleton through a mechanism involving these proteins.

Key words: Cytokinesis, Microtubules, RhoA, Centralspindlin, ECT2

Related articles in JCS:

RhoA ploughs a furrow

JCS 2006 119: 101. [Full Text]  

This article has been cited by other articles:

				K. Murthy and P. Wadsworth Dual role for microtubules in regulating cortical contractility during cytokinesis J. Cell Sci., July 15, 2008; 121(14): 2350 - 2359. [Abstract] [Full Text] [PDF]

				P. P. D'Avino, T. Takeda, L. Capalbo, W. Zhang, K. S. Lilley, E. D. Laue, and D. M. Glover Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site J. Cell Sci., April 15, 2008; 121(8): 1151 - 1158. [Abstract] [Full Text] [PDF]

				J. K. Baruni, E. M. Munro, and G. von Dassow Cytokinetic furrowing in toroidal, binucleate and anucleate cells in C. elegans embryos J. Cell Sci., February 1, 2008; 121(3): 306 - 316. [Abstract] [Full Text] [PDF]

				M. Kanada, A. Nagasaki, and T. Q.P. Uyeda Novel Functions of Ect2 in Polar Lamellipodia Formation and Polarity Maintenance during "Contractile Ring-Independent" Cytokinesis in Adherent Cells Mol. Biol. Cell, January 1, 2008; 19(1): 8 - 16. [Abstract] [Full Text] [PDF]

				V. Pavicic-Kaltenbrunner, M. Mishima, and M. Glotzer Cooperative Assembly of CYK-4/MgcRacGAP and ZEN-4/MKLP1 to Form the Centralspindlin Complex Mol. Biol. Cell, December 1, 2007; 18(12): 4992 - 5003. [Abstract] [Full Text] [PDF]

				G. Margall-Ducos, S. Celton-Morizur, D. Couton, O. Bregerie, and C. Desdouets Liver tetraploidization is controlled by a new process of incomplete cytokinesis J. Cell Sci., October 15, 2007; 120(20): 3633 - 3639. [Abstract] [Full Text] [PDF]

				T. Kamasaki, M. Osumi, and I. Mabuchi Three-dimensional arrangement of F-actin in the contractile ring of fission yeast J. Cell Biol., August 27, 2007; 178(5): 765 - 771. [Abstract] [Full Text] [PDF]

				K. J. C. Verbrugghe and J. G. White Cortical centralspindlin and G{alpha} have parallel roles in furrow initiation in early C. elegans embryos J. Cell Sci., May 15, 2007; 120(10): 1772 - 1778. [Abstract] [Full Text] [PDF]

				M. E. Burkard, C. L. Randall, S. Larochelle, C. Zhang, K. M. Shokat, R. P. Fisher, and P. V. Jallepalli Chemical genetics reveals the requirement for Polo-like kinase 1 activity in positioning RhoA and triggering cytokinesis in human cells PNAS, March 13, 2007; 104(11): 4383 - 4388. [Abstract] [Full Text] [PDF]

				P. P. D'Avino, M. S. Savoian, L. Capalbo, and D. M. Glover RacGAP50C is sufficient to signal cleavage furrow formation during cytokinesis J. Cell Sci., November 1, 2006; 119(21): 4402 - 4408. [Abstract] [Full Text] [PDF]

				R. B. Chalamalasetty, S. Hummer, E. A. Nigg, and H. H. W. Sillje Influence of human Ect2 depletion and overexpression on cleavage furrow formation and abscission J. Cell Sci., July 15, 2006; 119(14): 3008 - 3019. [Abstract] [Full Text] [PDF]