spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 23 May 2006
doi: 10.1242/jcs.02970

Journal of Cell Science 119, 2445-2456 (2006)
Published by The Company of Biologists 2006
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow All Versions of this Article:
jcs.02970v1
119/12/2445    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McQuade, K. J.
Right arrow Articles by Rapraeger, A. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McQuade, K. J.
Right arrow Articles by Rapraeger, A. C.

Research Article

Syndecan-1 regulates {alpha}vß5 integrin activity in B82L fibroblasts

K. J. McQuade1,*, D. M. Beauvais2,*, B. J. Burbach2 and A. C. Rapraeger1,2,3,{ddagger}

1 Graduate Programs in Cellular and Molecular Biology, University of Wisconsin-Madison, Madison, WI 53706, USA
2 Graduate Programs in Molecular and Cellular Pharmacology, University of Wisconsin-Madison, Madison, WI 53706, USA
3 Graduate Programs in Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA

{ddagger} Author for correspondence (e-mail: acraprae{at}wisc.edu)

Accepted 7 March 2006

B82L mouse fibroblasts respond to fibronectin or vitronectin via a syndecan-1-mediated activation of the {alpha}vß5 integrin. Cells attached to syndecan-1-specific antibody display only filopodial extension. However, the syndecan-anchored cells extend lamellipodia when the antibody-substratum is supplemented with serum, or low concentrations of adsorbed vitronectin or fibronectin, that are not sufficient to activate the integrin when plated alone. Integrin activation is blocked by treatment with (Arg-Gly-Asp)-containing peptides and function-blocking antibodies that target {alpha}v integrins, as well as by siRNA-mediated silencing of ß5 integrin expression. In addition, {alpha}vß5-mediated cell attachment and spreading on high concentrations of vitronectin is blocked by competition with recombinant syndecan-1 ectodomain core protein and by downregulation of mouse syndecan-1 expression by mouse-specific siRNA. Taking advantage of the species-specificity of the siRNA, rescue experiments in which human syndecan-1 constructs are expressed trace the activation site to the syndecan-1 ectodomain. Moreover, both full-length mouse and human syndecan-1 co-immunoprecipitate with the ß5 integrin subunit, but fail to do so if the syndecan is displaced by competition with soluble, recombinant syndecan-1 ectodomain. These results suggest that the ectodomain of the syndecan-1 core protein contains an active site that assembles into a complex with the {alpha}vß5 integrin and regulates {alpha}vß5 integrin activity.

Key words: Syndecan-1, {alpha}vß5 integrin, Cell adhesion, Extracellular matrix, Vitronectin, Proteoglycan




This article has been cited by other articles:


Home page
 Cardiovasc ResHome page
M. Murakami, A. Elfenbein, and M. Simons
Non-canonical fibroblast growth factor signalling in angiogenesis
Cardiovasc Res, May 1, 2008; 78(2): 223 - 231.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
M. Gotte, D. Spillmann, G. W. Yip, E. Versteeg, F. G. Echtermeyer, T. H. van Kuppevelt, and L. Kiesel
Changes in heparan sulfate are associated with delayed wound repair, altered cell migration, adhesion and contractility in the galactosyltransferase I (ss4GalT-7) deficient form of Ehlers-Danlos syndrome
Hum. Mol. Genet., April 1, 2008; 17(7): 996 - 1009.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Y. Yang, V. MacLeod, Y. Dai, Y. Khotskaya-Sample, Z. Shriver, G. Venkataraman, R. Sasisekharan, A. Naggi, G. Torri, B. Casu, et al.
The syndecan-1 heparan sulfate proteoglycan is a viable target for myeloma therapy
Blood, September 15, 2007; 110(6): 2041 - 2048.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. A. Stepp, Y. Liu, S. Pal-Ghosh, R. A. Jurjus, G. Tadvalkar, A. Sekaran, K. LoSicco, L. Jiang, M. Larsen, L. Li, et al.
Reduced migration, altered matrix and enhanced TGFbeta1 signaling are signatures of mouse keratinocytes lacking Sdc1
J. Cell Sci., August 15, 2007; 120(16): 2851 - 2863.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. A. Kirkpatrick and S. B. Selleck
Heparan sulfate proteoglycans at a glance
J. Cell Sci., June 1, 2007; 120(11): 1829 - 1832.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Su, S. A. Blaine, D. Qiao, and A. Friedl
Shedding of Syndecan-1 by Stromal Fibroblasts Stimulates Human Breast Cancer Cell Proliferation via FGF2 Activation
J. Biol. Chem., May 18, 2007; 282(20): 14906 - 14915.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
P. Ma, S. L. Beck, R. W. Raab, R. L. McKown, G. L. Coffman, A. Utani, W. J. Chirico, A. C. Rapraeger, and G. W. Laurie
Heparanase deglycanation of syndecan-1 is required for binding of the epithelial-restricted prosecretory mitogen lacritin
J. Cell Biol., September 25, 2006; 174(7): 1097 - 1106.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006