spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online June 20, 2006
doi: 10.1242/10.1242/jcs.03020

Journal of Cell Science 119, 2837-2846 (2006)
Published by The Company of Biologists 2006
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dovas, A.
Right arrow Articles by Couchman, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dovas, A.
Right arrow Articles by Couchman, J. R.

Research Article

PKC{alpha}-dependent activation of RhoA by syndecan-4 during focal adhesion formation

Athanassios Dovas, Atsuko Yoneda and John R. Couchman*

Division of Biomedical Sciences, Imperial College London, London, SW7 2AZ, UK

* Author for correspondence (e-mail: j.couchman{at}imperial.ac.uk)

Accepted 19 April 2006

Syndecan-4 is a ubiquitously expressed transmembrane heparan sulphate proteoglycan acting in concert with integrins in the formation of focal adhesions and stress fibres. Signalling events studied thus far suggest the formation of a ternary complex between syndecan-4, phosphatidylinositol 4,5-bisphosphate and protein kinase C {alpha} (PKC{alpha}). Syndecan-4 clustering at the cell surface has also been associated with RhoA-dependent signalling, but the relationship between PKC{alpha} and RhoA has not been resolved. Here we present evidence that syndecan-4, PKC{alpha} and RhoA are in a linear pathway necessary for the formation and maintenance of stress fibres in primary rat embryo fibroblasts. Inhibition of PKC{alpha} activity through the use of specific pharmacological inhibitors, a dominant-negative construct, or siRNA downregulation of protein levels, attenuated focal adhesion formation and the maintenance of stress fibres. However, these effects could be bypassed through independent activation of RhoA with lysophosphatidic acid, but not by clustering of syndecan-4 with ligand. Furthermore, inhibition of PKC{alpha} could block the increase in the GTP levels of RhoA induced by clustering of syndecan-4 at the cell surface. All these data point to a mechanism whereby syndecan-4 signals to RhoA in a PKC{alpha}-dependent manner and PKC{alpha} directly influences RhoA activity.

Key words: Syndecan-4, PKC{alpha}, RhoA, Fibronectin, Stress fibres, Focal adhesions




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
S. Huveneers, H. Truong, R. Fassler, A. Sonnenberg, and E. H. J. Danen
Binding of soluble fibronectin to integrin {alpha}5{beta}1 - link to focal adhesion redistribution and contractile shape
J. Cell Sci., August 1, 2008; 121(15): 2452 - 2462.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Telci, Z. Wang, X. Li, E. A. M. Verderio, M. J. Humphries, M. Baccarini, H. Basaga, and M. Griffin
Fibronectin-Tissue Transglutaminase Matrix Rescues RGD-impaired Cell Adhesion through Syndecan-4 and {beta}1 Integrin Co-signaling
J. Biol. Chem., July 25, 2008; 283(30): 20937 - 20947.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Z. Sun, L. A. Martinez-Lemus, M. A. Hill, and G. A. Meininger
Extracellular matrix-specific focal adhesions in vascular smooth muscle produce mechanically active adhesion sites
Am J Physiol Cell Physiol, July 1, 2008; 295(1): C268 - C278.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
M. D. Bass, M. R. Morgan, K. A. Roach, J. Settleman, A. B. Goryachev, and M. J. Humphries
p190RhoGAP is the convergence point of adhesion signals from {alpha}5{beta}1 integrin and syndecan-4
J. Cell Biol., June 16, 2008; 181(6): 1013 - 1026.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
M. Gotte, D. Spillmann, G. W. Yip, E. Versteeg, F. G. Echtermeyer, T. H. van Kuppevelt, and L. Kiesel
Changes in heparan sulfate are associated with delayed wound repair, altered cell migration, adhesion and contractility in the galactosyltransferase I (ss4GalT-7) deficient form of Ehlers-Danlos syndrome
Hum. Mol. Genet., April 1, 2008; 17(7): 996 - 1009.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. D. Dubash, K. Wennerberg, R. Garcia-Mata, M. M. Menold, W. T. Arthur, and K. Burridge
A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA activity downstream of adhesion to fibronectin
J. Cell Sci., November 15, 2007; 120(22): 3989 - 3998.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. A. Kirkpatrick and S. B. Selleck
Heparan sulfate proteoglycans at a glance
J. Cell Sci., June 1, 2007; 120(11): 1829 - 1832.
[Full Text] [PDF]

Home page
 J. Cell Biol.Home page
M. D. Bass, K. A. Roach, M. R. Morgan, Z. Mostafavi-Pour, T. Schoen, T. Muramatsu, U. Mayer, C. Ballestrem, J. P. Spatz, and M. J. Humphries
Syndecan-4-dependent Rac1 regulation determines directional migration in response to the extracellular matrix
J. Cell Biol., May 7, 2007; 177(3): 527 - 538.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. V. Bax, Y. Mahalingam, S. Cain, K. Mellody, L. Freeman, K. Younger, C. A. Shuttleworth, M. J. Humphries, J. R. Couchman, and C. M. Kielty
Cell adhesion to fibrillin-1: identification of an Arg-Gly-Asp-dependent synergy region and a heparin-binding site that regulates focal adhesion formation
J. Cell Sci., April 15, 2007; 120(8): 1383 - 1392.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006