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First published online 27 June 2006
doi: 10.1242/jcs.03013
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Research Article |
1 and TRß differentially regulate gene expression of Kcnq4 and prestin during final differentiation of outer hair cells
1 University of Tübingen, Department of Otolaryngology, Tübingen Hearing Research Centre (THRC), Laboratory of Molecular Neurobiology, Elfriede-Aulhorn-Str. 5, 72076 Tübingen, Germany
2 University of Tübingen, Institute of Physiology II and Department of Otolaryngology, THRC, Gmelinstr. 5, 72076 Tübingen, Germany
3 Max-Planck-Institute for Experimental Endocrinology, Feodor-Lynen-Str. 7, 30625 Hannover, Germany
4 Developmental Auditory Physiology Laboratory, Boys Town National Research Hospital, 555 North 30th Street, Omaha, NE 68131, USA
* Author for correspondence (e-mail: marlies.knipper{at}uni-tuebingen.de)
Accepted 6 April 2006
Thyroid hormone (TH or T3) and TH-receptor ß (TRß) have been reported to be relevant for cochlear development and hearing function. Mutations in the TRß gene result in deafness associated with resistance to TH syndrome. The effect of TR
1 on neither hearing function nor cochlear T3 target genes has been described to date. It is also uncertain whether TR1 and TRß can act simultaneously on different target genes within a single cell. We focused on two concomitantly expressed outer hair cell genes, the potassium channel Kcnq4 and the motor protein prestin Slc26a5. In outer hair cells, TH enhanced the expression of the prestin gene through TRß. Simultaneously Kcnq4 expression was activated in the same cells by derepression of TR1 aporeceptors mediated by an identified THresponse element, which modulates KCNQ4 promoter activity. We show that T3 target genes can differ in their sensitivity to TH receptors having the ligand either bound (holoreceptors) or not bound (aporeceptors) within single cells, and suggest a role for TR1 in final cell differentiation.
Key words: KCNQ4, Prestin, Final differentiation, TR aporeceptors
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