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First published online 22 August 2006
doi: 10.1242/jcs.03146

Journal of Cell Science 119, 3764-3775 (2006)
Published by The Company of Biologists 2006
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Research Article

Golgi structural stability and biogenesis depend on associated PKA activity

Eloy Bejarano*, Margarita Cabrera*, Lucia Vega, Josefina Hidalgo and Angel Velasco{ddagger}

Department of Cell Biology, Faculty of Biology, University of Seville, Avd. Reina Mercedes s/n, 41012 Seville, Spain

{ddagger} Author for correspondence (e-mail: avelasco{at}us.es)

Accepted 20 June 2006

The mammalian Golgi complex consists of stacks of cisternae linked laterally into a continuous perinuclear ribbon structure. Protein kinase A is stably associated with the Golgi complex during interphase. To analyze its role in Golgi structural maintenance cells were depleted of protein kinase A regulatory subunits using small interfering RNAs. Under these conditions, the catalytic subunits redistributed to the cytosol and the entire Golgi complex underwent disassembly into multiple juxtanuclear fragments. A similar effect took place following pharmacological inhibition or redistribution of the complete holoenzyme to the cytosol. Golgi fragments maintained their polarization and competence for anterograde protein trafficking. By electron microscopy, they were identified as whorl-like structures composed of concentrically arrayed cisternae. To test a possible role of protein kinase A in Golgi biogenesis we analyzed its involvement during Golgi reassembly from the endoplasmic reticulum. In cells incubated with protein kinase A inhibitors, Golgi reconstruction was arrested at a late step of the reassembly process. This is consistent with the stage of enzyme recruitment from cytosol to emerging Golgi membranes during the reassembly process. We conclude that protein kinase A activity plays a relevant role in the assembly and maintenance of a continuous Golgi ribbon from separated membrane stacks.

Key words: Golgi complex, Protein kinase A, AKAP




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