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First published online 12 September 2006
doi: 10.1242/jcs.03182

Journal of Cell Science 119, 4025-4032 (2006)
Published by The Company of Biologists 2006
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Research Article

Characterization of a novel meiosis-specific protein within the central element of the synaptonemal complex

Geert Hamer1, Katarina Gell1, Anna Kouznetsova1, Ivana Novak1, Ricardo Benavente2 and Christer Höög1,*

1 Department of Cell and Molecular Biology, Karolinska Institute, Berzelius väg 35, Stockholm, SE-171 77, Sweden
2 Department of Cell and Developmental Biology, Biocenter of the University of Würzburg, Am Hubland, Würzburg 97074, Germany

* Author for correspondence (e-mail: Christer.Hoog{at}ki.se)

Accepted 24 July 2006

During the first meiotic prophase, alignment and synapsis of the homologous chromosomes are mediated by the synaptonemal complex. Incorrect assembly of this complex results in cell death, impaired meiotic recombination and formation of aneuploid germ cells. We have identified a novel mouse meiosis-specific protein, TEX12, and shown it to be a component of the central element structure of the synaptonemal complex at synapsed homologous chromosomes. Only two other central element proteins, SYCE1 and SYCE2, have been identified to date and, using several mouse knockout models, we show that these proteins and TEX12 specifically depend on the synaptonemal transverse filament protein SYCP1 for localization to the meiotic chromosomes. Additionally, we show that TEX12 exactly co-localized with SYCE2, having the same, often punctate, localization pattern. SYCE1, on the other hand, co-localized with SYCP1 and these proteins displayed the same more continuous expression pattern. These co-localization studies were confirmed by co-immunoprecipitation experiments that showed that TEX12 specifically co-precipitated with SYCE2. Our results suggest a molecular network within the central elements, in which TEX12 and SYCE2 form a complex that interacts with SYCE1. SYCE1 interacts more directly with SYCP1 and could thus anchor the central element proteins to the transverse filaments.

Key words: Meiosis, Synaptonemal complex, Central element proteins


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