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First published online 17 October 2006
doi: 10.1242/jcs.03218

Journal of Cell Science 119, 4541-4553 (2006)
Published by The Company of Biologists 2006
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Research Article

Podoplanin binds ERM proteins to activate RhoA and promote epithelial-mesenchymal transition

Ester Martín-Villar1, Diego Megías2, Susanna Castel3, Maria Marta Yurrita1, Senén Vilaró3,* and Miguel Quintanilla1,{ddagger}

1 Instituto de Investigaciones Biomédicas Alberto Sols, Consejo Superior de Investigaciones Científicas (CSIC)-Universidad Autónoma de Madrid (UAM), 28029 Madrid, Spain
2 Centro Nacional de Investigaciones Oncológicas, 28029 Madrid, Spain
3 Departamento de Biología Celular, Universidad de Barcelona, 08028 Barcelona, Spain

{ddagger} Author for correspondence (e-mail: mquintanilla{at}iib.uam.es)

Accepted 9 August 2006

Podoplanin is a small membrane mucin expressed in tumors associated with malignant progression. It is enriched at cell-surface protrusions where it colocalizes with members of the ERM (ezrin, radixin, moesin) protein family. Here, we found that human podoplanin directly interacts with ezrin (and moesin) in vitro and in vivo through a cluster of basic amino acids within its cytoplasmic tail, mainly through a juxtamembrane dipeptide RK. Podoplanin induced an epithelial-mesenchymal transition in MDCK cells linked to the activation of RhoA and increased cell migration and invasiveness. Fluorescence time-lapse video observations in migrating cells indicate that podoplanin might be involved in ruffling activity as well as in retractive processes. By using mutant podoplanin constructs fused to green fluorescent protein we show that association of the cytoplasmic tail with ERM proteins is required for upregulation of RhoA activity and epithelial-mesenchymal transition. Furthermore, expression of either a dominant-negative truncated variant of ezrin or a dominant-negative mutant form of RhoA blocked podoplanin-induced RhoA activation and epithelial-mesenchymal transition. These results provide a mechanistic basis to understand the role of podoplanin in cell migration or invasiveness.

Key words: Podoplanin/PA2.26 antigen, Ezrin, RhoA, Epithelial-mesenchymal transition, Cell migration




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© The Company of Biologists Ltd 2006