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First published online 7 November 2006
doi: 10.1242/jcs.03274

Journal of Cell Science 119, 4811-4818 (2006)
Published by The Company of Biologists 2006
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Short Report

A systematic RNA interference screen reveals a cell migration gene network in C. elegans

Erin J. Cram, Hongyu Shang and Jean E. Schwarzbauer*

Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014, USA

* Author for correspondence (e-mail: jschwarz{at}princeton.edu)

Accepted 21 September 2006

Summary

Cell migration is essential during embryonic development and tissue morphogenesis. During gonadogenesis in the nematode Caenorhabditis elegans, migration of the distal tip cells forms two U-shaped gonad arms. Malformation results if the distal tip cells stop prematurely or follow an aberrant path, and abnormalities are easily visualized in living nematodes. Here we describe the first comprehensive in vivo RNA interference screen for genes required for cell migration. In this non-biased screen, we systematically analyzed 16,758 RNA-interference depletion experiments by light microscopy and identified 99 genes required for distal tip cell migration. Genetic and physical interaction data connect 59 of these genes to form a cell migration gene network that defines distal tip cell migration in vivo.

Key words: Cell migration, Caenorhabditis elegans, RNAi, Gonad


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© The Company of Biologists Ltd 2006