spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 14 November 2006
doi: 10.1242/jcs.03276

Journal of Cell Science 119, 4889-4900 (2006)
Published by The Company of Biologists 2006
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow All Versions of this Article:
jcs.03276v1
119/23/4889    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JCS
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sagolla, M. S.
Right arrow Articles by Cande, W. Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sagolla, M. S.
Right arrow Articles by Cande, W. Z.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Three-dimensional analysis of mitosis and cytokinesis in the binucleate parasite Giardia intestinalis

Meredith S. Sagolla, Scott C. Dawson*, Joel J. Mancuso and W. Zacheus Cande{ddagger}

345 LSA, Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA 94720, USA

{ddagger} Author for correspondence (e-mail: zcande{at}berkeley.edu)

Accepted 22 September 2006

In the binucleate parasite Giardia intestinalis, two diploid nuclei and essential cytoskeletal structures including eight flagella are duplicated and partitioned into two daughter cells during cell division. The mechanisms of mitosis and cytokinesis in the binucleate parasite Giardia are poorly resolved, yet have important implications for the maintenance of genetic heterozygosity. To articulate the mechanism of mitosis and the plane of cell division, we used three-dimensional deconvolution microscopy of each stage of mitosis to monitor the spatial relationships of conserved cytological markers to the mitotic spindles, the centromeres and the spindle poles. Using both light- and transmission electron microscopy, we determined that Giardia has a semi-open mitosis with two extranuclear spindles that access chromatin through polar openings in the nuclear membranes. In prophase, the nuclei migrate to the cell midline, followed by lateral chromosome segregation in anaphase. Taxol treatment results in lagging chromosomes and half-spindles. Our analysis supports a nuclear migration model of mitosis with lateral chromosome segregation in the left-right axis and cytokinesis along the longitudinal plane (perpendicular to the spindles), ensuring that each daughter inherits one copy of each parental nucleus with mirror image symmetry. Fluorescence in situ hybridization (FISH) to an episomal plasmid confirms that the nuclei remain separate and are inherited with mirror image symmetry.

Key words: Giardia intestinalis, Mitosis, Cytokinesis, Spindle, Basal body, Centromere


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related articles in JCS:

Double trouble in Giardia mitosis

JCS 2006 119: 2304. [Full Text]  



This article has been cited by other articles:


Home page
 Eukaryot CellHome page
M. L. Carpenter and W. Z. Cande
Using Morpholinos for Gene Knockdown in Giardia intestinalis
Eukaryot. Cell, June 1, 2009; 8(6): 916 - 919.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
D. M. Ratner, J. Cui, M. Steffen, L. L. Moore, P. W. Robbins, and J. Samuelson
Changes in the N-Glycome, Glycoproteins with Asn-Linked Glycans, of Giardia lamblia with Differentiation from Trophozoites to Cysts
Eukaryot. Cell, November 1, 2008; 7(11): 1930 - 1940.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. C. Hoeng, S. C. Dawson, S. A. House, M. S. Sagolla, J. K. Pham, J. J. Mancuso, J. Lowe, and W. Z. Cande
High-Resolution Crystal Structure and In Vivo Function of a Kinesin-2 Homologue in Giardia intestinalis
Mol. Biol. Cell, July 1, 2008; 19(7): 3124 - 3137.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. K. Poxleitner, S. C. Dawson, and W. Z. Cande
Cell Cycle Synchrony in Giardia intestinalis Cultures Achieved by Using Nocodazole and Aphidicolin
Eukaryot. Cell, April 1, 2008; 7(4): 569 - 574.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
M. K. Poxleitner, M. L. Carpenter, J. J. Mancuso, C.-J. R. Wang, S. C. Dawson, and W. Z. Cande
Evidence for Karyogamy and Exchange of Genetic Material in the Binucleate Intestinal Parasite Giardia intestinalis
Science, March 14, 2008; 319(5869): 1530 - 1533.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
S. C. Dawson, M. S. Sagolla, J. J. Mancuso, D. J. Woessner, S. A. House, L. Fritz-Laylin, and W. Z. Cande
Kinesin-13 Regulates Flagellar, Interphase, and Mitotic Microtubule Dynamics in Giardia intestinalis
Eukaryot. Cell, December 1, 2007; 6(12): 2354 - 2364.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
R. Williams
W. Zacheus Cande: Evolutionary biologist in cell biologist's clothing
J. Cell Biol., September 7, 2007; 178(5): 718 - 719.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
S. Teodorovic, J. M. Braverman, and H. G. Elmendorf
Unusually Low Levels of Genetic Variation among Giardia lamblia Isolates
Eukaryot. Cell, August 1, 2007; 6(8): 1421 - 1430.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006