QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 14 February 2006
doi: 10.1242/jcs.02785

This Article Figures Only Full Text Full Text (PDF) Supplementary Material All Versions of this Article: jcs.02785v1 119/5/807    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Anitei, M. Articles by Pfeiffer, S. E. Search for Related Content PubMed PubMed Citation Articles by Anitei, M. Articles by Pfeiffer, S. E. Social Bookmarking                         What's this?

A role for Sec8 in oligodendrocyte morphological differentiation

¹ Program of Molecular Biology and Biochemistry, University of Connecticut Medical School, Farmington, CT 06030, USA
² Department of Neuroscience, University of Connecticut Medical School, Farmington, CT 06030, USA
³ Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow, G12 8QQ, UK

^* Author for correspondence (e-mail: pfeiffer{at}neuron.uchc.edu)

Accepted 7 November 2005

In the central nervous system, oligodendrocytes synthesize vast amounts of myelin, a multilamellar membrane wrapped around axons that dramatically enhances nerve transmission. A complex apparatus appears to coordinate trafficking of proteins and lipids during myelin synthesis, but the molecular interactions involved are not well understood. We demonstrate that oligodendrocytes express several key molecules necessary for the targeting of transport vesicles to areas of rapid membrane growth, including the exocyst components Sec8 and Sec6 and the multidomain scaffolding proteins CASK and Mint1. Sec8 overexpression significantly promotes oligodendrocyte morphological differentiation and myelin-like membrane formation in vitro; conversely, siRNA-mediated interference with Sec8 expression inhibits this process, and anti-Sec8 antibody induces a reduction in oligodendrocyte areas. In addition, Sec8 colocalizes, coimmunoprecipitates and cofractionates with the major myelin protein OSP/Claudin11 and with CASK in oligodendrocytes. These results suggest that Sec8 plays a central role in oligodendrocyte membrane formation by regulating the recruitment of vesicles that transport myelin proteins such as OSP/Claudin11 to sites of membrane growth.

Key words: Exocyst, Oligodendrocyte, Myelin

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Related articles in JCS:

Exocyst implicated in myelin wrap

JCS 2006 119: 503. [Full Text]  

This article has been cited by other articles:

				G. Lalli RalA and the exocyst complex influence neuronal polarity through PAR-3 and aPKC J. Cell Sci., May 15, 2009; 122(10): 1499 - 1506. [Abstract] [Full Text] [PDF]

				K. S. Spiczka and C. Yeaman Ral-regulated interaction between Sec5 and paxillin targets Exocyst to focal complexes during cell migration J. Cell Sci., September 1, 2008; 121(17): 2880 - 2891. [Abstract] [Full Text] [PDF]

				C. Winterstein, J. Trotter, and E.-M. Kramer-Albers Distinct endocytic recycling of myelin proteins promotes oligodendroglial membrane remodeling J. Cell Sci., March 15, 2008; 121(6): 834 - 842. [Abstract] [Full Text] [PDF]

				M. Simons and K. Trajkovic Neuron-glia communication in the control of oligodendrocyte function and myelin biogenesis J. Cell Sci., November 1, 2006; 119(21): 4381 - 4389. [Abstract] [Full Text] [PDF]