QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 21 February 2006
doi: 10.1242/jcs.02829

This Article Figures Only Full Text Full Text (PDF) All Versions of this Article: jcs.02829v1 119/6/1063    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by He, S. Articles by Davie, J. R. Search for Related Content PubMed PubMed Citation Articles by He, S. Articles by Davie, J. R. Social Bookmarking                         What's this?

Sp1 and Sp3 foci distribution throughout mitosis

Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, R3E 0V9, Canada

^* Author for correspondence (e-mail: davie{at}cc.umanitoba.ca)

Accepted 6 December 2005

The mammalian transcription factors Sp1 and Sp3 compete for the same DNA binding sites but play different roles in the regulation of expression of numerous genes. It is known that, in the interphase nucleus, Sp1 and Sp3 are organized into distinct foci. In this study, we show that throughout the mitotic process, while being displaced from the condensed chromosomes and dispersed throughout the cell, Sp1 and Sp3 maintain their separate punctate distributions. In metaphase, both Sp1 and Sp3 foci show a high degree of colocalization with microfilaments, suggesting that F-actin is involved in the organization of Sp1 and Sp3 foci during mitosis. Constant Sp1 and Sp3 levels were observed during mitosis, signifying a recovery of the pre-existing Sp1 and Sp3 population in newly formed nuclei. In late telophase, Sp1 and Sp3 are equally segregated between daughter cells, and their subnuclear organization as distinct foci is restored in a sequential fashion with Sp3 regrouping into the newly formed nuclei prior to Sp1. Both Sp1 and Sp3 return to the nuclei ahead of RNA polymerase II. Our results support a model in which entry of Sp1, Sp3 and RNA polymerase II into the newly formed nuclei is an ordered process.

Key words: Sp1, Sp3, Transcription factors, Mitosis, Cytoskeleton

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				G. N. Corry, M. J. Hendzel, and D. A. Underhill Subnuclear localization and mobility are key indicators of PAX3 dysfunction in Waardenburg syndrome Hum. Mol. Genet., June 15, 2008; 17(12): 1825 - 1837. [Abstract] [Full Text] [PDF]

				S. Cherukuri, R. Hock, T. Ueda, F. Catez, M. Rochman, and M. Bustin Cell Cycle-dependent Binding of HMGN Proteins to Chromatin Mol. Biol. Cell, May 1, 2008; 19(5): 1816 - 1824. [Abstract] [Full Text] [PDF]

				J.-Y. Chuang, Y.-T. Wang, S.-H. Yeh, Y.-W. Liu, W.-C. Chang, and J.-J. Hung Phosphorylation by c-Jun NH2-terminal Kinase 1 Regulates the Stability of Transcription Factor Sp1 during Mitosis Mol. Biol. Cell, March 1, 2008; 19(3): 1139 - 1151. [Abstract] [Full Text] [PDF]